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IL-33 Antibody (700268) in WB
Western blot was performed using IL-33 Recombinant Rabbit Monoclonal Antibody (13H20L1) (Product # 700268) and bands at 19 and 31 kDa corresponding to IL-33 was observed in Mouse Lung and Mouse Ovary. Whole cell extracts (30 µg lysate) of Mouse Lung (Lane 1), Mouse Ovary (Lane 2), and Mouse Bone Marrow (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21... View More
产品信息
700268
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
13H20L1
抗原
A recombinant protein corresponding to amino acids 109-266 of mouse IL-33.
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2532311
产品详细信息
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
IL-33 (Interleukin-33) is a 270 amino acid, highly divergent protein belonging to the IL-1 family with an IL-1-like C-terminal domain. IL-33 is a dual function protein that may function both as a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. IL-33 binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6. IL-33 activates NF-kappaB and MAP kinases, and drives production of TH2-associated cytokines from in vitro polarized TH2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. IL-33 is proteolytically converted to a mature form by CASP1 and is highly expressed in high endothelial venules found in tonsils, Peyer's patches and mesenteric lymph nodes and is almost undetectable in placenta. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE. Studies have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine, and an intracellular nuclear factor with transcriptional regulatory properties.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: H-IL-33; IL-33; ILN; Interleukin; Interleukin-1 receptor-like 1; Interleukin-33; Interleukin-33 receptor alpha chain; Interleukin33; Lymphocyte antigen 84; nuclear factor from high endothelial venules; Protein ST2; Protein T1; RP11-575C20.2
基因别名: 9230117N10Rik; DER4; Fit-1; Il-33; Il1f11; Il1rl1; Il33; Ly84; NF-HEV; St2; St2-rs1; ST2L; Ste2; T1; T1/ST2
UniProt ID: (Mouse) P14719, (Mouse) Q8BVZ5
Entrez Gene ID: (Mouse) 17082, (Mouse) 77125
negative regulation of T-helper 1 type immune response
signal transduction
negative regulation of cell proliferation
negative regulation of interferon-gamma production
positive regulation of interleukin-5 production
interleukin-33-mediated signaling pathway
positive regulation of macrophage activation
negative regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of inflammatory response
positive regulation of chemokine secretion
negative regulation of leukocyte migration
positive regulation of type 2 immune response
transcription, DNA-templated
positive regulation of gene expression
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
positive regulation of interleukin-13 production
positive regulation of interleukin-4 production
positive regulation of interleukin-6 production
positive regulation of transcription from RNA polymerase II promoter
positive regulation of immunoglobulin secretion
negative regulation of immunoglobulin secretion
defense response to virus
extrinsic apoptotic signaling pathway
