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IL-33R (ST2) Antibody (17-9338-42) in Flow
LAD2 cells were stained with Mouse IgG1 kappa Isotype Control, APC (Product # 17-4714-82) (blue histogram) or IL-33R (ST2) Monoclonal Antibody, APC (purple histogram). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).
产品信息
17-9338-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Not Applicable
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
hIL33Rcap
抗原
Extracellular domain of human IL-33R protein
偶联物
激发/发射光谱
651/660 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2762446
产品详细信息
Description: This hIL33Rcap monoclonal antibody recognizes human IL-33R, also known as ST2. This antibody binds both, the membrane bound form (ST2L) and the soluble form (ST2S). This antibody is suitable for both surface and intracellular staining.
This hIL33Rcap clone is also used as a capture antibody in the IL-33R (ST2) Human ProcartaPlex™ Simplex Kit (Product # EPX010-12231-901)
Applications Reported: This hIL33Rcap antibody has been reported for use in flow cytometric analysis.
Applications Tested: This hIL33Rcap antibody has been pre-diluted and tested by flow cytometric analysis of LAD2 cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
靶标信息
IL-33R, also known as ST2, is a receptor for IL-33. It is a member of the IL-1 receptor family of innate receptors. Its extracellular domain consists of 3 Ig-like domains that directly interact with its co-receptor IL-1RAcP, and the ligand alarmin, IL-33. IL-33 ligation results in the association of IL-33R complex with its adaptor proteins MyD88, IRAK1, IRAK4 and TRAF6, and activation of the NF-kB, and MAPK pathways. This, in turn, instigates the release of chemokines and cytokines such as IL-5, IL-6, IL-8, and IL-13. Two most common isoforms of IL-33R that result from alternative splicing are ST2L - the membrane bound form, and ST2S - the soluble form. The membrane bound form can be released from the cell surface by proteolytic cleavage. The soluble forms have been suggested to act as decoys dampening the IL-33 signaling. IL-33R is primarily expressed by mast cells, basophils, and eosinophils. In mouse, IL-33R expression has been shown on ILC2 and Th2 cells. IL-33R signaling has been implicated in the number of immune conditions, including allergies, arthritis, atherosclerosis and sepsis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
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生物信息学
蛋白别名: growth stimulation-expressed; homolog of mouse growth stimulation-expressed; IL-1 R4; IL-1RL1; IL-33R; interleukin 1 receptor-like 1; interleukin 1 receptor-related protein; Interleukin-1 receptor-like 1; Interleukin1 receptor like 1; Protein ST2; sIL 33R; soluble IL 33 Receptor; soluble IL 33R
基因别名: DER4; FIT-1; IL1RL1; IL33R; ST2; ST2L; ST2V; T1
UniProt ID: (Human) Q01638
Entrez Gene ID: (Human) 9173
negative regulation of T-helper 1 type immune response
immune response
signal transduction
negative regulation of cell proliferation
negative regulation of interferon-gamma production
positive regulation of interleukin-5 production
interleukin-33-mediated signaling pathway
positive regulation of macrophage activation
negative regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of inflammatory response
positive regulation of chemokine secretion
cellular response to peptide
