Invitrogen

IL-5 Polyclonal Antibody

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IL-5 Antibody (PA5-96761) in WB

Western blot analysis of IL-5 in extracts of various cell lines. Samples were incubated with IL-5 Polyclonal antibody (Product # PA5-96761) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 15s.

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产品信息

PA5-96761

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

1:50-1:200

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 20-134 of human IL5 (NP_0008701)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

6.11 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2808563

产品详细信息

Immunogen sequence: IPTEIPTSAL VKETLALLST HRTLLIANET LRIPVPVHKN HQLCTEEIFQ GIGTLESQTV QGGTVERLFK NLSLIKKYID GQKKKCGEER RRVNQFLDYL QEFLGVMNTE WIIES; Positive Samples: Mouse thymus, Mouse liver, Rat thymus

靶标信息

IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Group 2 innate lymphoid cells protect mouse heart from myocardial infarction injury via interleukin 5, eosinophils, and dendritic cells.

Cardiovascular research

Liu T,Meng Z,Liu J,Li J,Zhang Y,Deng Z,Luo S,Wang M,Huang Q,Zhang S,Fendt P,Devouassoux J,Li D,McKenzie ANJ,Nahrendorf M,Libby P,Guo J,Shi GP

PA5-96761 was used in Immunohistochemistry to reveal an indirect cardiac reparative role of ILC2 in post-MI hearts via the IL5, EOS, and DC mechanism.

Tue May 02 00:00:00 UTC 2023

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

PA5-96761 was used in Immunohistochemistry to establish both direct and indirect roles for ILC2 in peri-aortic CaPO4 injury-induced mouse abdominal aortic aneurysms.

Wed Mar 01 00:00:00 UTC 2023

生物信息学

蛋白别名: B-cell differentiation factor I; B-cell growth factor II; BCGF-II; colony-stimulating factor, eosinophil; Cytotoxic T-lymphocyte inducer; Eosinophil differentiation factor; IL-5; ILN; Interleukin; Interleukin 5 (colony-stimulating factor eosinophil); Interleukin 5 (colony-stimulating factor, eosinophil); Interleukin-5; Interleukin5; T-cell replacing factor; TRF

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基因别名: EDF; IL-5; IL5; TRF

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UniProt ID: (Human) P05113, (Mouse) P04401

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Entrez Gene ID: (Human) 3567, (Rat) 24497, (Mouse) 16191

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功能

protein tyrosine kinase activity Ras guanyl-nucleotide exchange factor activity cytokine activity interleukin-5 receptor binding protein binding growth factor activity

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