Invitrogen

IL-5 Monoclonal Antibody (TRFK5)

产品信息

MA5-23690

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

2.5 µg per million cells

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酶联免疫吸附实验 (ELISA)

0.5-2 µg/mL (Detection), 2-8 µg/mL (Capture)

中和实验 (Neu)

0.004-0.015 µg/mL

阻断反应 (BLOCK)

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产品规格

种属反应

Human, Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG1

分类

Monoclonal

类型

Antibody

克隆号

TRFK5

抗原

Mouse T cell clone-derived partially purified recombinant mouse IL-5

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.5 mg/mL

纯化类型

Protein A/G

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2609713

产品详细信息

In sandwich ELISAs, no cross-reactivity with recombinant human (rh) IL-3, rhGM-CSF, recombinant mouse (rm) IL-5, rhIL-5, rmIL-3, or rmGM-CSF is observed.

Reconstitute at 0.5 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

靶标信息

IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A mechanism for the induction of type 2 immune responses by a protease allergen in the genital tract.

Proceedings of the National Academy of Sciences of the United States of America

Oh JE,Oh DS,Jung HE,Lee HK

MA523690 was used in blocking or activating experiment to propose that type 2 immunity is induced by a unique mechanism in the genital tract

Tue Feb 14 00:00:00 UTC 2017

Role of arginase 1 from myeloid cells in th2-dominated lung inflammation.

PloS one

Barron L,Smith AM,El Kasmi KC,Qualls JE,Huang X,Cheever A,Borthwick LA,Wilson MS,Murray PJ,Wynn TA

MA5-23690 was used in flow cytometry to determine the contribution of Arg1 to lung inflammation and pathophysiology

Mon Dec 09 00:00:00 UTC 2013

Influence of mucosal adjuvants on antigen passage and CD4+ T cell activation during the primary response to airborne allergen.

Journal of immunology (Baltimore, Md. : 1950)

Wikstrom ME,Batanero E,Smith M,Thomas JA,von Garnier C,Holt PG,Stumbles PA

MA5-23690 was used in flow cytometry to study the effects of Escherichia coli LPS and cholera toxin on antigen passage and T cell activation following nasal administration OVA

Sat Jul 15 00:00:00 UTC 2006

生物信息学

蛋白别名: B-cell differentiation factor I; B-cell growth factor II; BCGF-II; colony-stimulating factor, eosinophil; Cytotoxic T-lymphocyte inducer; Eosinophil differentiation factor; IL-5; ILN; Interleukin; Interleukin-5; Interleukin5; T-cell replacing factor; TRF

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基因别名: EDF; IL-5; IL5; TRF

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UniProt ID: (Human) P05113, (Mouse) P04401

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Entrez Gene ID: (Human) 3567, (Mouse) 16191

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功能

protein tyrosine kinase activity Ras guanyl-nucleotide exchange factor activity cytokine activity interleukin-5 receptor binding protein binding growth factor activity

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