Invitrogen

IL-6 Polyclonal Antibody, Biotin, PeproTech®

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IL-6 Antibody (500-P56BT-25UG) in WB

Western Blot: To detect Murine IL-6 by Western Blot analysis IL-6 Polyclonal Antibody, Biotin (Product # 500-P56BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine IL-6 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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产品信息

500-P56BT-25UG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

酶联免疫吸附实验 (ELISA)

0.25-1.0 µg/mL

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产品规格

种属反应

Mouse

已发表种属

Bacteria, Mouse

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Murine IL-6

查看免疫原

偶联物

Biotin Biotin Biotin

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

AB_2930633

产品详细信息

AA Sequence of recombinant protein: MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKELCNGN SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKI SSGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-6. Anti-Murine IL-6-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Murine IL-6 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IL-6 (500-P56) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IL-6.

Western Blot: To detect Murine IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine IL-6 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P56BT-1MG will be provided as 2 x 500 µg

靶标信息

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

仅用于科研。不用于诊断过程。未经明确授权不得转售。