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IL-6 Antibody (710042) in ELISA
Direct ELISA analysis of IL-6 was performed by coating wells of a 96-well plate with 100 µL per well of IL-6 (Product # RIL6I) diluted in carbonate/bicarbonate buffer (Product # 28382) at a concentration of 2 µg/mL overnight at 4C. Wells of the plate were washed, blocked with starting blocking buffer (Product # 37538), and incubated with 100 µL per well of a rabbit Anti-IL-6 Recombinant Rabbit Superclonal™ Antibody (Product # 710042) at a serially dilution of 1:50, 1:100, 1:250, 1:500, 1:1000, 1:2000 and 1:5000 for 90 minutes at 37C. The p... View More
产品信息
710042
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
克隆号
4HCLC
抗原
Protein corresponding to amino acids 30-212 of human IL-6
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2532536
产品详细信息
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
靶标信息
IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.
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生物信息学
蛋白别名: B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; H-IL-6; Hybridoma growth factor; IFN-beta-2; IL-6; ILN; Interferon beta-2; Interleukin; interleukin BSF-2; Interleukin-6; Interleukin6; M-IL-6; R-IL-6
基因别名: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; IL6
UniProt ID: (Human) P05231
Entrez Gene ID: (Human) 3569
neutrophil apoptotic process
response to yeast
hepatic immune response
neutrophil mediated immunity
monocyte chemotaxis
positive regulation of acute inflammatory response
positive regulation of leukocyte chemotaxis
negative regulation of protein kinase activity
acute-phase response
inflammatory response
humoral immune response
aging
positive regulation of cell proliferation
negative regulation of cell proliferation
regulation of cell shape
response to heat
response to cold
regulation of vascular endothelial growth factor production
positive regulation of gene expression
negative regulation of lipid storage
response to auditory stimulus
cell growth
cytokine-mediated signaling pathway
platelet activation
response to caffeine
endocrine pancreas development
neuron projection development
response to nutrient levels
response to peptidoglycan
positive regulation of chemokine production
positive regulation of interleukin-6 production
response to insulin
negative regulation of collagen biosynthetic process
positive regulation of peptidyl-serine phosphorylation
positive regulation of protein import into nucleus, translocation
cellular response to hepatocyte growth factor stimulus
positive regulation of T cell proliferation
response to drug
positive regulation of tyrosine phosphorylation of Stat3 protein
glucose homeostasis
defense response to protozoan
positive regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
response to amino acid
positive regulation of MAPK cascade
negative regulation of chemokine biosynthetic process
regulation of circadian sleep/wake cycle, non-REM sleep
positive regulation of nitric oxide biosynthetic process
cell redox homeostasis
negative regulation of fat cell differentiation
positive regulation of T-helper 2 cell differentiation
positive regulation of neuron differentiation
positive regulation of osteoblast differentiation
negative regulation of gluconeogenesis
positive regulation of translation
positive regulation of DNA replication
regulation of angiogenesis
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JAK-STAT cascade
response to antibiotic
muscle cell cellular homeostasis
bone remodeling
negative regulation of hormone secretion
negative regulation of muscle organ development
positive regulation of smooth muscle cell proliferation
positive regulation of epithelial cell proliferation
negative regulation of cytokine secretion
positive regulation of peptidyl-tyrosine phosphorylation
defense response to Gram-negative bacterium
defense response to Gram-positive bacterium
positive regulation of B cell activation
positive regulation of immunoglobulin secretion
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of NF-kappaB transcription factor activity
response to glucocorticoid
response to calcium ion
response to electrical stimulus
defense response to virus
positive regulation of protein kinase B signaling
positive regulation of transmission of nerve impulse
branching involved in salivary gland morphogenesis
epithelial cell proliferation involved in salivary gland morphogenesis
glucagon secretion
interleukin-6-mediated signaling pathway
cellular response to hydrogen peroxide
positive regulation of ERK1 and ERK2 cascade
cellular response to lipopolysaccharide
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to dexamethasone stimulus
positive regulation of cell proliferation in bone marrow
T-helper 17 cell lineage commitment
negative regulation of neuron death
positive regulation of STAT protein import into nucleus
positive regulation of type B pancreatic cell apoptotic process
