Invitrogen

IL-7 Polyclonal Antibody, PeproTech®

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IL-7 Antibody (500-P310-1MG) in WB

Western Blot: To detect Rat IL-7 by Western Blot analysis IL-7 Polyclonal Antibody (Product # 500-P310-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat IL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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产品信息

500-P310-1MG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

酶联免疫吸附实验 (ELISA)

0.5-2.0 µg/mL

产品规格

种属反应

Rat

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Rat IL-7

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

AB_2930330

产品详细信息

AA Sequence of recombinant protein: MDCHIKDKDG KAFGSVLMIS INQLDKMTGT DSDCPNNEPN FFKKHLCDDT KEAAFLNRAA RKLRQFLKMN ISEEFNDHLL RVSDGTQTLV NCTSKEEKTI KEQKKNDPCF LKRLLREIKT CWNKILKGSI.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat IL-7. Anti-Rat IL-7-specific antibody was purified by affinity chromatography employing an immobilized Rat IL-7 matrix.

Sandwich ELISA: To detect Rat IL-7 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat IL-7 (500-P310Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-7.

Western Blot: To detect Rat IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat IL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

靶标信息

Interleukin 7 (IL-7) is a hematopoietic cytokine that is constitutively produced by stromal cells from the bone marrow and thymus. IL-7 signals through the IL-7R to promote the differentiation of hematopoietic stem cells to lymphoid precursor cells, which give rise to T cells, B cells, and natural killer cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。