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IL-8 (CXCL8) Antibody (PA5-47289) in ICC/IF
Immunocytochemistry analysis of IL-8 (CXCL8) in immersion fixed feline peripheral blood mononuclear cells (PBMCs). Samples were incubated in IL-8 (CXCL8) polyclonal antibody (Product # PA5-47289) using a dilution of 15 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to... View More
产品信息
PA5-47289
产品规格
种属反应
Cat
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived recombinant feline CXCL8/IL-8 Ala23-Ala101
偶联物
形式
Lyophilized
浓度
0.2 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5% trehalose
内含物
No Preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2576462
产品详细信息
In direct ELISAs, approximately 40% cross-reactivity with recombinant canine IL-8 is observed and 15% cross-reactivity with recombinant porcine IL-8 and recombinant human IL-8 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
靶标信息
Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor which belongs to the CXC subfamily of chemokines, and are bound by the cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and potent angiogenic factor. The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. IL-8 and ten other members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours, and IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ANX7; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; IL-8; ILN; Interleukin; Interleukin-8; Interleukin8; RP11-537A6.8; SNX; SYNEXIN
基因别名: CXCL8; IL8
UniProt ID: (Cat) Q9XSX5
Entrez Gene ID: (Cat) 493836
