Invitrogen

IL-8 (CXCL8) Monoclonal Antibody (I8-S2), Biotin

图: 1 / 9

IL-8 (CXCL8) Antibody (M802B) in ELISA

Sandwich ELISA analysis of human IL-8 was performed using a Human IL-8 Colorimetric ELISA kit (Product # EH2IL8) by loading 50 µL per well of Human IL-8 Recombinant Protein (Product # SIL8) in dodecuplicate at 1000, 400, 160, 64, 25.6, and 0 pg/mL across a 3 µg/mL mouse anti-Human IL-8 (Product # M801) pre-coated plate and incubating for 1 hour at room temperature. The plate was washed, and then incubated with 50 µL per well of a biotinylated mouse anti-human IL-8 monoclonal antibody (Product # M802B) in duplicate at 0.25 µg/mL for 1 hour ... View More

IL-8 (CXCL8) Antibody in Radioimmune assays (RIA)

Product Image Gallery

产品信息

M802B

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

酶联免疫吸附实验 (ELISA)

Assay-dependent

查看17篇已发表文章 17篇已发表文章

放射免疫测定 (RIA)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

I8-S2

抗原

Recombinant human IL-8

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein G

保存液

PBS with 4% BSA

内含物

proprietary Preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_223584

产品详细信息

M802B targets IL-8 in ELISA, and WB applications and shows reactivity with Human samples.

The M802B immunogen is recombinant human IL-8.

M802B detects IL-8 which has a predicted molecular weight of approximately 9 kDa.

The M802B IL8 antibody (biotinylated conjugate of clone I8-S2) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M801 (clone 3IL8-H10). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Biotinylated antibody M802B (clone I8-S2) and antibody M801 (clone 3IL8-H10) have successfully been used in combination with recombinant IL8 protein SIL8 in ELISA applications.

靶标信息

Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor which belongs to the CXC subfamily of chemokines, and are bound by the cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and potent angiogenic factor. The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. IL-8 and ten other members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours, and IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Macrophage migration inhibitory factor regulates specific innate immune sensor responses in gingival epithelial cells.

Journal of periodontology

Kantrong N,Chang AM,Bamashmous S,Hajjar AM,Bucala RJ,Darveau RP

M802B was used in Enzyme-linked immunosorbent assay to demonstrate that MIF is a significant contributor to the maintenance of healthy oral homeostasis.

Thu Dec 01 00:00:00 UTC 2022

Chronic effects of two rutile TiO₂ nanomaterials in human intestinal and hepatic cell lines.

Particle and fibre toxicology

Jalili P,Krause BC,Lanceleur R,Burel A,Jungnickel H,Lampen A,Laux P,Luch A,Fessard V,Hogeveen K

M802B was used in Enzyme-linked immunosorbent assay to indicate that although no clear effects on cytotoxicity were observed following repeated exposure of differentiated Caco-2 and HepaRG cells to TiO2 NMs, subtle effects on membrane composition could induce potential adverse effects in the long-term.

Tue May 17 00:00:00 UTC 2022

Comparative assessment of commercially available wound gels in ex vivo human skin reveals major differences in immune response-modulatory effects.

Scientific reports

Seiser S,Cerbu D,Gallhofer A,Matiasek J,Elbe-Bürger A

M802B was used in ELISA to demonstrate totally different effects for several commercially available hydrogels in our wound model, which gives also new insight into their tissue compatibility and mode of action.

Wed Oct 19 00:00:00 UTC 2022

Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin.

Scientific reports

Seiser S,Janker L,Zila N,Mildner M,Rakita A,Matiasek J,Bileck A,Gerner C,Paulitschke V,Elbe-Bürger A

M802B was used in Enzyme-linked immunosorbent assay to give insights on the mode of action based on which OCT positively influences wound healing and identified anti-inflammatory and protease-inhibitory activities of OCT.

Fri Jan 08 00:00:00 UTC 2021

Combined effects of okadaic acid and pectenotoxin-2, 13-desmethylspirolide C or yessotoxin in human intestinal Caco-2 cells.

Chemosphere

Alarcan J,Barbé S,Kopp B,Hessel-Pras S,Braeuning A,Lampen A,Le Hégarat L,Fessard V

M802B was used in ELISA to examine the effects of three binary mixtures, OA/PTX-2, OA/SPX-1 and OA/YTX on human intestinal Caco-2 cells.

Thu Aug 01 00:00:00 UTC 2019

Inflammatory cytokines tumor necrosis factor-α, interleukin-8 and sleep monitoring in patients with obstructive sleep apnea syndrome.

Experimental and therapeutic medicine

Ming H,Tian A,Liu B,Hu Y,Liu C,Chen R,Cheng L

M802B was used in an ELISA assay to investigate the changes of tumour necrosis factor-α, interleukin-8 and sleep ability in patients with obstructive sleep apnea hypopnea syndrome.

Fri Mar 01 00:00:00 UTC 2019

Resolvin D2 decreases TLR4 expression to mediate resolution in human monocytes.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Croasdell A,Sime PJ,Phipps RP

Published figure using IL-8 (CXCL8) monoclonal antibody (Product # M802B) in ELISA

Thu Sep 01 00:00:00 UTC 2016

The human salivary proteome is radiation responsive.

Radiation research

Moore HD,Ivey RG,Voytovich UJ,Lin C,Stirewalt DL,Pogosova-Agadjanyan EL,Paulovich AG

Published figure using IL-8 (CXCL8) monoclonal antibody (Product # M802B) in Radioimmune Assays

Thu May 01 00:00:00 UTC 2014

Microparticles engineered to highly express peroxisome proliferator-activated receptor-γ decreased inflammatory mediator production and increased adhesion of recipient monocytes.

PloS one

Sahler J,Woeller CF,Phipps RP

Published figure using IL-8 (CXCL8) monoclonal antibody (Product # M802B) in ELISA

Mon Jul 13 00:00:00 UTC 2015

Attenuation of inflammatory mediator production by the NF-κB member RelB is mediated by microRNA-146a in lung fibroblasts.

American journal of physiology. Lung cellular and molecular physiology

McMillan DH,Woeller CF,Thatcher TH,Spinelli SL,Maggirwar SB,Sime PJ,Phipps RP

Published figure using IL-8 (CXCL8) monoclonal antibody (Product # M802B) in ELISA

Sat Jun 01 00:00:00 UTC 2013

Transforming growth factor-β impairs glucocorticoid activity in the A549 lung adenocarcinoma cell line.

British journal of pharmacology

Salem S,Harris T,Mok JS,Li MY,Keenan CR,Schuliga MJ,Stewart AG

M802B was used in ELISA to investigate the effect of transforming growth factor-beta on glucocorticoid activity in human A549 cells

Wed Aug 01 00:00:00 UTC 2012

Optical protein sensor for detecting cancer markers in saliva.

Biosensors & bioelectronics

Tan W,Sabet L,Li Y,Yu T,Klokkevold PR,Wong DT,Ho CM

M802B was used in ELISA to develop a new sensor for cancer detection in saliva

Wed Oct 15 00:00:00 UTC 2008

Optical protein sensor for detecting cancer markers in saliva.

Biosensors & bioelectronics

Tan W,Sabet L,Li Y,Yu T,Klokkevold PR,Wong DT,Ho CM

M802B was used in ELISA to develop a new sensor for cancer detection in saliva

Wed Oct 15 00:00:00 UTC 2008

IgE modulates neutrophil survival in asthma: role of mitochondrial pathway.

Journal of immunology (Baltimore, Md. : 1950)

Saffar AS,Alphonse MP,Shan L,Hayglass KT,Simons FE,Gounni AS

M802B was used in ELISA to study the role of mitochondrial pathway in neutrophil survival

Thu Feb 15 00:00:00 UTC 2007

Protein microarray platform for the multiplex analysis of biomarkers in human sera.

Journal of immunological methods

Urbanowska T,Mangialaio S,Zickler C,Cheevapruk S,Hasler P,Regenass S,Legay F

M802B was used in ELISA to develop a protein microarray platform for the multiplex analysis of biomarkers in human sera

Fri Oct 20 00:00:00 UTC 2006

Oxidized ATP (oATP) attenuates proinflammatory signaling via P2 receptor-independent mechanisms.

British journal of pharmacology

Beigi RD,Kertesy SB,Aquilina G,Dubyak GR

M802B was used in ELISA to study the role of oxidized ATP in proinflammatory signaling

Wed Oct 01 00:00:00 UTC 2003

Development of protein microarray technology to monitor biomarkers of rheumatoid arthritis disease.

Cell biology and toxicology

Urbanowska T,Mangialaio S,Hartmann C,Legay F

M802B was used in ELISA to develop protein microarray technology to monitor biomarkers of rheumatoid arthritis disease

Sun Jun 01 00:00:00 UTC 2003

Monocyte chemoattractant protein-1 levels in bronchoalveolar lavage fluid of lung-transplanted patients treated with tacrolimus as rescue treatment for refractory acute rejection.

Transplantation proceedings

Meloni F,Cascina A,Paschetto E,Marone Bianco A,Morosini M,Pellegrini C,Fietta A,Vitulo P,Pozzi E,Viganò M

M802B was used in ELISA to investigate the effect of immunosuppressive regimens on cytokine expression in lung transplanted patients

Sun Jun 01 00:00:00 UTC 2003

Regulation of interleukin 8 expression in human malignant melanoma cells.

Cancer research

Singh RK,Varney ML

M802B was used in ELISA to investigate the regulation of interleukin 8 expression in melanoma cells

Wed Apr 01 00:00:00 UTC 1998

生物信息学

蛋白别名: (Ala-IL-8)77; (Ser-IL-8)72; alveolar macrophage chemotactic factor I; ANX7; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; GCP-1; GCP/IL-8 protein I; GCP/IL-8 protein II; GCP/IL-8 protein III; GCP/IL-8 protein IV; GCP/IL-8 protein V; GCP/IL-8 protein VI; Granulocyte chemotactic protein 1; IL-8; IL-8(1-77); IL-8(5-77); IL-8(6-77); IL-8(7-77); IL-8(8-77); IL-8(9-77); IL8/NAP1 form I; IL8/NAP1 form II; IL8/NAP1 form III; IL8/NAP1 form IV; IL8/NAP1 form V; IL8/NAP1 form VI; ILN; Interleukin; interleukin 8; Interleukin-8; Interleukin8; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; Lymphocyte-derived neutrophil-activating factor; LYNAP; MDNCF; MDNCF-a; MDNCF-b; MDNCF-c; MONAP; Monocyte-derived neutrophil chemotactic factor; Monocyte-derived neutrophil-activating peptide; NAF; NAP-1; Neutrophil-activating factor; neutrophil-activating peptide 1; Neutrophil-activating protein 1; Protein 3-10C; RP11-537A6.8; small inducible cytokine subfamily B, member 8; SNX; SYNEXIN; T-cell chemotactic factor; tumor necrosis factor-induced gene 1

查看更多收起

基因别名: CXCL8; GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1

查看更多收起

UniProt ID: (Human) P10145

查看更多收起

Entrez Gene ID: (Human) 3576

查看更多收起

功能

interleukin-8 receptor binding protein binding chemokine activity

参与通路