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ILK Antibody (PA5-115421) in ICC/IF
Immunocytochemistry analysis of ILK in HEPG2 cells. Samples were treated with PFA, permeabilized in 0.1% saponin prior to blocking in 10% serum (45 min at 37°C), and incubated with polyclonal antibody (Product # PA5-115421) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with Alexa Fluor 594 conjugated goat anti-rabbit IgG using a dilution of 1:600.
产品信息
PA5-115421
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human ILK(Accession Q13418), corresponding to amino acid residues H420-K452.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2900057
产品详细信息
Antibody detects endogenous levels of total ILK.
靶标信息
Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 59 kDa serine/threonine-protein kinase; Beta-integrin-linked kinase; epididymis secretory protein Li 28; ILK-1; ILK-2; integrin binding protein kinase; Integrin linked ILK; integrin linked kinase; integrin-linked kinase; integrin-linked kinase-2; Integrin-linked protein kinase; p59ILK
基因别名: AA511515; ESTM24; HEL-S-28; ILK; ILK-1; ILK-2; ILK1; ILK2; P59; p59ILK
UniProt ID: (Human) Q13418, (Mouse) O55222, (Rat) Q99J82
Entrez Gene ID: (Human) 3611, (Mouse) 16202, (Rat) 170922
protein serine/threonine kinase activity
signal transducer activity
integrin binding
protein binding
ATP binding
SH3 domain binding
protein kinase binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein domain specific binding
non-receptor serine/threonine protein kinase
branching involved in ureteric bud morphogenesis
positive regulation of cell-matrix adhesion
outflow tract morphogenesis
protein phosphorylation
negative regulation of protein kinase activity
cell cycle arrest
cell-matrix adhesion
integrin-mediated signaling pathway
cell aging
cell proliferation
positive regulation of cell proliferation
negative regulation of cardiac muscle cell apoptotic process
fibroblast migration
negative regulation of smooth muscle cell migration
peptidyl-serine phosphorylation
nerve development
myelination in peripheral nervous system
positive regulation of cell migration
positive regulation of BMP signaling pathway
myelin assembly
regulation of actin cytoskeleton organization
cell junction assembly
substrate adhesion-dependent cell spreading
positive regulation of phosphorylation
extracellular fibril organization
positive regulation of MAP kinase activity
protein kinase B signaling
negative regulation of neuron apoptotic process
establishment or maintenance of epithelial cell apical/basal polarity
positive regulation of myoblast differentiation
positive regulation of osteoblast differentiation
positive regulation of axon extension
positive regulation of transcription, DNA-templated
negative regulation of smooth muscle cell proliferation
neuron projection morphogenesis
positive regulation of dendrite morphogenesis
protein heterooligomerization
positive regulation of protein kinase B signaling
platelet aggregation
positive regulation of canonical Wnt signaling pathway
negative regulation of neural precursor cell proliferation
regulation of cell growth
regulation of signal transduction
Schwann cell development
phosphorylation
cell projection organization
negative regulation of apoptotic process
positive regulation of MAPK cascade
positive regulation of axonogenesis
