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IMPDH2 Antibody (PA5-27519) in ICC/IF
Immunofluorescence analysis of IMPDH2 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with IMPDH2 Polyclonal antibody (Product # PA5-27519) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27... View More
Product Details
PA5-27519
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 276 and 506 of Human IMPDH2
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7, with 40% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2544995
Product Specific Information
Recommended positive controls: HepG2, NIH-3T3, JC, BCL-1.
Predicted reactivity: Mouse (100%), Rat (99%), Zebrafish (94%), Xenopus laevis (96%), Chicken (96%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: IMP (inosine 5'-monophosphate) dehydrogenase 2; IMP (inosine monophosphate) dehydrogenase 2; IMP dehydrogenase 2; IMP dehydrogenase II; IMP dehydrogenase type II; IMP oxireductase 2; IMPD 2; IMPDH 2; IMPDH II; IMPDH-II; IMPDH2; inosine 5' phosphate dehydrogenase 2; inosine monophosphate dehydrogenase type II; Inosine-5'-monophosphate dehydrogenase 2; Inosine-5'-monophosphate dehydrogenase type II
Gene Aliases: IMPD; IMPD2; IMPDH-II; IMPDH2
UniProt ID: (Human) P12268, (Mouse) P24547
Entrez Gene ID: (Human) 3615, (Mouse) 23918
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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