Invitrogen

INCENP Monoclonal Antibody (58-217)

INCENP Antibody in Immunocytochemistry (ICC/IF)

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INCENP Antibody (39-2800) in ICC/IF

Immunofluorescence analysis of INCENP was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with INCENP Mouse Monoclonal Antibody (Product # 39-2800) at 5µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) a d... View More

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产品信息

39-2800

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

查看4篇已发表文章 4篇已发表文章

免疫细胞化学 (ICC/IF)

5 µg/mL

查看4篇已发表文章 4篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

58-217

抗原

Recombinant human INCENP (inner centromere protein)

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533405

靶标信息

Chromosomal passenger proteins are members of a group of proteins that move from centromeres to the spindle midzone during mitosis. INCENP (Inner Centromere Protein) is a chromosomal passenger protein with an essential role in mitosis and meiosis. It is a complex highly basic multidomain protein that is dynamically translocated from metaphase chromosomes to the spindle midzone during or just prior to anaphase. Some INCENP transfers to the equatorial cortex before formation of the cleavage furrow. Ultimately, it is discarded in the midbody at the completion of cytokinesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Transcription factor Sp1 regulates mitotic chromosome assembly and segregation.

Chromosoma

Flashner S,Swift M,Sowash A,Fahmy AN,Azizkhan-Clifford J

39-2800 was used in Western Blotting to demonstrate that the ubiquitously expressed transcription factor specificity protein 1 (Sp1), which we have previously linked to aneuploidy, has a mitosis-specific role regulating chromosome segregation.

Thu Sep 01 00:00:00 UTC 2022

MKLP2 Is a Motile Kinesin that Transports the Chromosomal Passenger Complex during Anaphase.

Current biology : CB

Adriaans IE,Hooikaas PJ,Aher A,Vromans MJM,van Es RM,Grigoriev I,Akhmanova A,Lens SMA

39-2800 was used in Western Blotting to indicate that control of cytokinesis initiation by the chromosomal passenger complex (CPC) requires its directional MKLP2-dependent transport.

Mon Jul 06 00:00:00 UTC 2020

CDK11^p58-cyclin L1β regulates abscission site assembly.

The Journal of biological chemistry

Renshaw MJ,Panagiotou TC,Lavoie BD,Wilde A

39-2800 was used in Immunocytochemistry-immunoflourescence to demonstrate that the mitosis-specific CDK11p58 kinase specifically forms a complex with cyclin L1β that, in late cytokinesis, localizes to the stem body, a structure in the middle of the intercellular bridge that forms between two dividing cells.

Fri Dec 06 00:00:00 UTC 2019

Baculoviral delivery of CRISPR/Cas9 facilitates efficient genome editing in human cells.

PloS one

Hindriksen S,Bramer AJ,Truong MA,Vromans MJM,Post JB,Verlaan-Klink I,Snippert HJ,Lens SMA,Hadders MA

39-2800 was used in Western Blotting to investigate the use of baculoviral vectors as a delivery vehicle for CRISPR/Cas9 based genome-editing tools.

Wed Sep 20 00:00:00 UTC 2017

Analysis of HDACi-Induced Changes in Chromosomal Passenger Complex Localization.

Methods in molecular biology (Clifton, N.J.)

Unruhe-Knauf B,Knauer SK

392800 was used in immunocytochemistry to analyze the localization of chromosomal passenger complex (CPC) after treatment with HDACi during cell division.

Tue Jan 09 00:00:00 UTC 2018

Ser2481-autophosphorylated mTOR colocalizes with chromosomal passenger proteins during mammalian cell cytokinesis.

Cell cycle (Georgetown, Tex.)

Vazquez-Martin A,Sauri-Nadal T,Menendez OJ,Oliveras-Ferraros C,Cufí S,Corominas-Faja B,López-Bonet E,Menendez JA

39-2800 was used in immunocytochemistry to study the colocalization of Ser2481-autophosphorylated mTOR and chromosomal passenger proteins during mammalian cell cytokinesis

Thu Nov 15 00:00:00 UTC 2012

Enhanced HSP70 lysine methylation promotes proliferation of cancer cells through activation of Aurora kinase B.

Nature communications

Cho HS,Shimazu T,Toyokawa G,Daigo Y,Maehara Y,Hayami S,Ito A,Masuda K,Ikawa N,Field HI,Tsuchiya E,Ohnuma S,Ponder BA,Yoshida M,Nakamura Y,Hamamoto R

39-2800 was used in western blot to report the dimethylation of HSP70 at Lys-561 by SETD1A

Fri Feb 01 00:00:00 UTC 2013

The active form of the metabolic sensor: AMP-activated protein kinase (AMPK) directly binds the mitotic apparatus and travels from centrosomes to the spindle midzone during mitosis and cytokinesis.

Cell cycle (Georgetown, Tex.)

Vazquez-Martin A,Oliveras-Ferraros C,Menendez JA

Sat Aug 01 00:00:00 UTC 2009

The active form of the metabolic sensor: AMP-activated protein kinase (AMPK) directly binds the mitotic apparatus and travels from centrosomes to the spindle midzone during mitosis and cytokinesis.

Cell cycle (Georgetown, Tex.)

Vazquez-Martin A,Oliveras-Ferraros C,Menendez JA

Sat Aug 01 00:00:00 UTC 2009

生物信息学

蛋白别名: binds and activates aurora-B and -C in vivo and in vitro; chromosomal passenger protein; Inner centromere protein; inner centromere protein antigens 135/155kDa; inner centromere protein INCENP

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基因别名: INCENP

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UniProt ID: (Human) Q9NQS7

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Entrez Gene ID: (Human) 3619

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功能

protein binding

参与通路

cytokinesis chromosome segregation sister chromatid cohesion mitotic nuclear division protein sumoylation