Invitrogen

INSR Recombinant Rabbit Monoclonal Antibody (23GB2050)

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抗体检测数据 (2)
高级验证 (1)

INSR Antibody in Western Blot (WB)

INSR Antibody in Western Blot (WB)

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INSR Antibody (MA5-53208) in WB

Western Blot analysis of INSR. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with INSR recombinant monoclonal antibody (Product # MA5-53208) with a dilution of 1:5,000, followed by HRP-conjugated goat anti-rabbit secondary antibody with a dilution of 1:20,000. Image was developed using FeQTM ECL Substrate Kit.

INSR Antibody in Western Blot (WB)

INSR Antibody in Flow Cytometry (Flow)

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INSR Antibody in Western Blot (WB) — Western Blot analysis of INSR. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with INSR recombinant monoclonal antibody (Product # MA5-53208) with a dilution of 1:5,000, followed by HRP-conjugated goat anti-rabbit secondary antibody with a dilution of 1:20,000. Image was developed using FeQTM ECL Substrate Kit.

INSR Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of INSR in HT-1080 cells using INSR recombinant monoclonal antibody (Product # MA5-53208) at a dilution of 1:2,000. Green, isotype control; red, INSR.

INSR Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Insulin receptor shRNA and decrease in signal intensity was observed in Western Blotting using Anti-INSR Recombinant Rabbit Monoclonal Antibody (23GB2050) (Product # MA5-53208) at a dilution of 1:5,000. Secondary antibody used was a goat anti-rabbit (HRP) at a dilution of 1:20,000. {KD}

产品信息

MA5-53208

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

-

流式细胞分析 (Flow)

1:2,000

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293F

分类

Recombinant Monoclonal

类型

Antibody

克隆号

23GB2050

抗原

A synthesized peptide derived from human Insulin Receptor (910-976AA).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

靶标信息

INSR is a receptor tyrosine kinase. Binding of insulin to the extracellular domain of INSR stimulates glucose uptake. INSR and IGF-1 receptors share major structural and functional similarity. The earliest cellular response to insulin stimulation is autophosphorylation of tyrosine in INSR. In humans, the INSR gene is located on chromosome 19. Defects in INSR are the cause of various insulin resistance syndromes and IGF-1R defects may also cause some forms of growth retardation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CD 220; CD220; CD220 antigen; CD220 beta; CD221; HHF 5; IGF-I receptor; Insulin receptor; insulin receptor preproprotein; IR; IR 1; IR beta; IR-1; IR1

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基因别名: 4932439J01Rik; CD220; D630014A15Rik; HHF5; INSR; IR; IR-A; IR-B

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UniProt ID: (Human) P06213, (Mouse) P15208

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Entrez Gene ID: (Human) 3643, (Rat) 24954, (Mouse) 16337

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protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity insulin-activated receptor activity insulin-like growth factor receptor binding protein binding ATP binding GTP binding protein phosphatase binding lipoic acid binding insulin-like growth factor I binding insulin-like growth factor II binding protein complex binding 3-phosphoinositide-dependent protein kinase binding phosphatidylinositol 3-kinase binding insulin binding insulin receptor substrate binding PTB domain binding protein kinase activity transmembrane receptor protein tyrosine kinase activity protein kinase binding protein domain specific binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups transmembrane signal receptor

activation of MAPK activity negative regulation of protein phosphorylation positive regulation of protein phosphorylation heart morphogenesis carbohydrate metabolic process regulation of transcription, DNA-templated G-protein coupled receptor signaling pathway positive regulation of cell proliferation insulin receptor signaling pathway epidermis development male gonad development response to manganese ion regulation of hydrogen peroxide metabolic process positive regulation of glycoprotein biosynthetic process negative regulation of gene expression response to activity peptidyl-tyrosine phosphorylation transformation of host cell by virus signal transduction by protein phosphorylation male sex determination adrenal gland development positive regulation of cell migration exocrine pancreas development activation of protein kinase activity activation of protein kinase B activity response to estradiol negative regulation of transporter activity cellular response to insulin stimulus response to vitamin D response to testosterone response to tumor necrosis factor peptidyl-tyrosine autophosphorylation glucose homeostasis positive regulation of MAPK cascade positive regulation of nitric oxide biosynthetic process fat cell differentiation response to ethanol positive regulation of glycogen biosynthetic process positive regulation of DNA replication positive regulation of glycolytic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated regulation of embryonic development positive regulation of glucose import protein autophosphorylation positive regulation of developmental growth protein heterotetramerization response to glucocorticoid positive regulation of meiotic cell cycle positive regulation of protein kinase B signaling positive regulation of respiratory burst cellular response to growth factor stimulus regulation of female gonad development protein phosphorylation transmembrane receptor protein tyrosine kinase signaling pathway response to hormone response to glucose organ morphogenesis response to nutrient levels response to insulin positive regulation of phosphorylation phosphorylation intracellular signal transduction