Invitrogen

INSR alpha Monoclonal Antibody (83-14)

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抗体检测数据 (4)

INSR alpha Antibody in Immunocytochemistry (ICC/IF)

INSR alpha Antibody in Immunocytochemistry (ICC/IF)

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INSR alpha Antibody (AHR0221) in ICC/IF

Immunofluorescence analysis of INSR alpha was performed using log phase IM-9 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with INSR alpha Monoclonal Antibody (83-14) (Product # AHR0221) at 5 µg/mL in 0.1% BSA, incubated overnight at 4 degree and then la... View More

INSR alpha Antibody in Immunocytochemistry (ICC/IF)

INSR alpha Antibody in Immunocytochemistry (ICC/IF)

INSR alpha Antibody in Western Blot (WB)

INSR alpha Antibody

Product Image Gallery

INSR alpha Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of INSR alpha was performed using log phase IM-9 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with INSR alpha Monoclonal Antibody (83-14) (Product # AHR0221) at 5 µg/mL in 0.1% BSA, incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

INSR alpha Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Insulin Receptor alpha (green) showing staining in the membrane of HepG2 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Insulin Receptor alpha monoclonal antibody (Product # AHR0221) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

INSR alpha Antibody in Western Blot (WB) — Western blot analysis was performed on membrane enriched extract (30 µg lysate) of IM9 (Lane 1). The blot was probed with Anti-INSR alpha Monoclonal Antibody (Product # AHR0221, 1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 130 kDa band corresponding to INSR alpha was observed in the cell line tested.

INSR alpha Antibody — Insulin Receptor (IR) and Insulin-like Growth Factor-1 Receptor (IGF1R) Protein Schematic-The insulin receptor (IR) is a receptor tyrosine kinase (RTK) that influences multiple signaling pathways through activation of a series of phosphorylation cascades. The IR is a heterotetrameric protein consisting of two ligand-binding a subunits and two b subunits that each contain a tyrosine kinase domain. Upon binding of insulin to the extracellular domain, the receptor is autophosphorylated to activate its intrinsic tyrosine kinase activity. The insulin-like growth factor-1 receptor (IGF1R) is similar to the IR, but exhibits a higher binding affinity for IGF-1 and activates a subset of distinct signaling pathways. Unique phosphorylation sites are said to contribute to signaling differences between IR and IGF1R, and may play a role in the different regulatory functions of the two proteins.

产品信息

AHR0221

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

-

免疫细胞化学 (ICC/IF)

5 µg/mL

-

酶联免疫吸附实验 (ELISA)

Assay-dependent

-

免疫沉淀 (IP)

2 µg / mg lysate

-

功能检测 (Functional)

Assay-dependent

-

抑制试剂 (IA)

Assay-dependent

-

产品规格

种属反应

Bovine, Human, Sheep, Pig

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

83-14

抗原

IM-9 lymphocytes followed by purified insulin receptor.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_2536349

产品详细信息

This antibody reacts with an epitope at aa 469-592 (exon 7/8). It primarily reacts with human, but also reacts very weakly with cow, pig and sheep.

This antibody can inhibit insulin binding (~80%). It can also be used in a Tyrosine Kinase assay (Ab-mediated capture on microtitre plates).

Without BSA, this antibody can be used as an insulin-like agonist. Without BSA, it can also be used as both a capture and detection antibody in a sandwich ELISA.

靶标信息

Biological actions of insulin and IGF-1 are mediated by their respective cell surface receptor tyrosine kinases that regulate multiple signaling pathways through activation of a series of phosphorylation cascades. The insulin receptor. Insulin/IGF-1 binding to the extracellular domain leads to autophosphorylation of downstream target proteins. These two receptors differ in sequence in regions that confer specificity for the designated ligand as well as in certain intracellular signaling domains, resulting in significant differences in the functional consequences of activation of each receptor. Defects in IR are the cause of various insulin resistance syndromes and IGF-1R defects may cause some forms of growth retardation. Both these signaling cascades are also important for the development of cancer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CD220; CD220 beta; Insulin receptor; IR; IR alpha; IR beta

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基因别名: CD220; HHF5; INSR; ir; ir-A

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UniProt ID: (Human) P06213

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Entrez Gene ID: (Bovine) 408017, (Human) 3643, (Sheep) 443431, (Pig) 396755

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protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity insulin-activated receptor activity insulin-like growth factor receptor binding protein binding ATP binding GTP binding protein phosphatase binding lipoic acid binding insulin-like growth factor I binding insulin-like growth factor II binding protein complex binding 3-phosphoinositide-dependent protein kinase binding phosphatidylinositol 3-kinase binding insulin binding insulin receptor substrate binding PTB domain binding transmembrane signal receptor

activation of MAPK activity negative regulation of protein phosphorylation positive regulation of protein phosphorylation heart morphogenesis carbohydrate metabolic process regulation of transcription, DNA-templated G-protein coupled receptor signaling pathway positive regulation of cell proliferation insulin receptor signaling pathway epidermis development male gonad development response to manganese ion regulation of hydrogen peroxide metabolic process positive regulation of glycoprotein biosynthetic process negative regulation of gene expression response to activity peptidyl-tyrosine phosphorylation transformation of host cell by virus signal transduction by protein phosphorylation male sex determination adrenal gland development positive regulation of cell migration exocrine pancreas development activation of protein kinase activity activation of protein kinase B activity response to estradiol negative regulation of transporter activity cellular response to insulin stimulus response to vitamin D response to testosterone response to tumor necrosis factor peptidyl-tyrosine autophosphorylation glucose homeostasis positive regulation of MAPK cascade positive regulation of nitric oxide biosynthetic process fat cell differentiation response to ethanol positive regulation of glycogen biosynthetic process positive regulation of DNA replication positive regulation of glycolytic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated regulation of embryonic development positive regulation of glucose import protein autophosphorylation positive regulation of developmental growth protein heterotetramerization response to glucocorticoid positive regulation of meiotic cell cycle positive regulation of protein kinase B signaling positive regulation of respiratory burst cellular response to growth factor stimulus regulation of female gonad development