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IPO5 Antibody (PA5-96023) in WB
Western blot analysis of IPO5 in mouse testis. Samples were incubated with IPO5 Polyclonal antibody (Product # PA5-96023) using a dilution of 1:400, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
产品信息
PA5-96023
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human IPO5 (NP_0022623)
偶联物
形式
Liquid
浓度
3.31 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2807825
产品详细信息
Immunogen sequence: AAAAAEQQQF YLLLGNLLSP DNVVRKQAEE TYENIPGQSK ITFLLQAIRN TTAAEEARQM AAVLLRRLLS SAFDEVYPAL PSDVQTAIKS ELLMIIQMET QSSMRKKVCD IAAELARNLI DEDGNNQWPE GLKFLFDSVS SQNVGLREAA LHIFWNFPGI FGNQQQHYLD VIKRMLVQCM QDQEHPSIRT LSARATAAFI LANEHNVALF KHFADLLPGF LQAVNDSCYQ NDDSVLKSLV EIADTVPKYL RPHLEATLQL SLKLCGDTSL NNMQRQLALE V; Positive Samples: SW620, HeLa, 293T, HepG2, Mouse testis, Mouse brain; Cellular Location: Cytoplasm, Nucleus, nucleolus
Excitation: 405 nm; Emission: 645 nm; Laser: Blue Laser
靶标信息
Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Imp5; importin 5; importin beta-3 subunit; Importin subunit beta-3; Importin-5; IPO 5; karyopherin (importin) beta 3; Karyopherin beta 3; Karyopherin beta-3; MGC2068; RAN binding protein 5; Ran-binding protein 5; Ran_GTP binding protein 5; RanBP 5; RanBP5
基因别名: 1110011C18Rik; 5730478E03Rik; AA409333; C76941; IMB3; imp5; IPO5; KPNB3; Pse1; RANBP5
UniProt ID: (Human) O00410, (Mouse) Q8BKC5
Entrez Gene ID: (Human) 3843, (Rat) 306182, (Mouse) 70572
protein import into nucleus, docking
protein import into nucleus, translocation
NLS-bearing protein import into nucleus
ribosomal protein import into nucleus
viral process
negative regulation of GTPase activity
positive regulation of protein import into nucleus
cellular response to amino acid stimulus
intracellular protein transport
transport
protein transport
