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IQGAP1 Antibody (PA5-95625) in ICC/IF
Immunocytochemistry analysis of IQGAP1 using anti-IQGAP1 antibody (Product # PA5-95625) . IQGAP1 was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-IQGAP1 antibody (Product # PA5-95625) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAP... View More
Product Details
PA5-95625
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human IQGAP1 recombinant protein (D1374-K1657).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2807427
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human placenta tissue, human U-87MG whole cell. IHC: human intestinal cancer tissue, human intestinal cancer tissue, human mammary cancer tissue, human mammary cancer tissue. ICC/IF: U20S cell. Flow: HepG2 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cdc42-Rac1 effector protein; DISP2; DISPB; p195; Ras GTPase-activating-like protein IQGAP1; RasGAP-like with IQ motifs
Gene Aliases: AA682088; D7Ertd237e; D7Ertd257e; HUMORFA01; IQGAP1; KIAA0051; mKIAA0051; p195; SAR1
UniProt ID: (Human) P46940, (Mouse) Q9JKF1
Entrez Gene ID: (Human) 8826, (Mouse) 29875, (Rat) 361598
GTPase inhibitor activity
GTPase activator activity
calcium ion binding
protein binding
calmodulin binding
phosphatidylinositol-3,4,5-trisphosphate binding
protein kinase binding
protein phosphatase binding
protein domain specific binding
protein complex binding
protein serine/threonine kinase activator activity
S100 protein binding
Rac GTPase binding
cadherin binding involved in cell-cell adhesion
Rho GTPase binding
regulation of cytokine production
signal transduction
epidermal growth factor receptor signaling pathway
small GTPase mediated signal transduction
fibroblast growth factor receptor signaling pathway
negative regulation of dephosphorylation
cellular response to platelet-derived growth factor stimulus
regulation of GTPase activity
cellular response to fibroblast growth factor stimulus
positive regulation of protein kinase activity
platelet-derived growth factor receptor signaling pathway
cellular response to calcium ion
cellular response to epidermal growth factor stimulus
positive regulation of protein serine/threonine kinase activity
neuron projection extension
negative regulation of GTPase activity
positive regulation of GTPase activity
glomerular visceral epithelial cell development
cell-cell adhesion
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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