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IQGAP1 Antibody (PA5-96385) in ICC/IF
Immunofluorescence analysis of IQGAP1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with IQGAP1 Polyclonal Antibody (Product # PA5-96385) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790... View More
Product Details
PA5-96385
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 500-750 of human IQGAP1 (NP_0038611)
Conjugate
Form
Liquid
Concentration
1.07 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2808187
Product Specific Information
Immunogen sequence: ELMKLKAQAH AENNEFITWN DIQACVDHVN LVVQEEHERI LAIGLINEAL DEGDAQKTLQ ALQIPAAKLE GVLAEVAQHY QDTLIRAKRE KAQEIQDESA VLWLDEIQGG IWQSNKDTQE AQKFALGIFA INEAVESGDV GKTLSALRSP DVGLYGVIPE CGETYHSDLA EAKKKKLAVG DNNSKWVKHW VKGGYYYYHN LETQEGGWDE PPNFVQNSMQ LSREEIQSSI SGVTAAYNRE QLWLANEGLI T; Positive Samples: A-549, 293T, HepG2, HT-29, HeLa; Cellular Location: Cell membrane
Target Information
This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cdc42-Rac1 effector protein; DISP2; DISPB; p195; Ras GTPase-activating-like protein IQGAP1; RasGAP-like with IQ motifs
Gene Aliases: AA682088; D7Ertd237e; D7Ertd257e; HUMORFA01; IQGAP1; KIAA0051; mKIAA0051; p195; SAR1
UniProt ID: (Human) P46940, (Mouse) Q9JKF1
Entrez Gene ID: (Human) 8826, (Mouse) 29875, (Rat) 361598
GTPase inhibitor activity
GTPase activator activity
calcium ion binding
protein binding
calmodulin binding
phosphatidylinositol-3,4,5-trisphosphate binding
protein kinase binding
protein phosphatase binding
protein domain specific binding
protein complex binding
protein serine/threonine kinase activator activity
S100 protein binding
Rac GTPase binding
cadherin binding involved in cell-cell adhesion
Rho GTPase binding
G-protein modulator
protein-binding activity modulator
GTPase-activating protein
regulation of cytokine production
signal transduction
epidermal growth factor receptor signaling pathway
fibroblast growth factor receptor signaling pathway
negative regulation of GTPase activity
negative regulation of dephosphorylation
cellular response to platelet-derived growth factor stimulus
positive regulation of GTPase activity
positive regulation of protein kinase activity
platelet-derived growth factor receptor signaling pathway
cellular response to calcium ion
cellular response to epidermal growth factor stimulus
positive regulation of protein serine/threonine kinase activity
glomerular visceral epithelial cell development
cell-cell adhesion
neuron projection extension
small GTPase mediated signal transduction
regulation of GTPase activity
cellular response to fibroblast growth factor stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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