Invitrogen

IRF3 Monoclonal Antibody (SL-12 (SL12)), eBioscience™

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IRF3 Antibody (14-9947-82) in WB

Western blot was performed using Anti-IRF3 Monoclonal Antibody (SL-12 (SL12)), eBioscience™ (Product # 14-9947-82) and a 47 kDa band corresponding to IRF3 was observed across cell lines tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of A549 (Lane 1), HCT 116 (Lane 2), HeLa (Lane 3), Jurkat (Lane 4), Ramos (Lane 5), K-562 (Lane 6) and THP-1 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by i... View More

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IRF3 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A549 cells were transfected with IRF3 siRNA and decrease in signal intensity was observed in western blot application using Anti-IRF3 Monoclonal Antibody (SL-12 (SL12)), eBioscience™ (Product # 14-9947-82). {KD}

产品信息

14-9947-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

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产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

SL-12 (SL12)

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_1272269

产品详细信息

Description: This SL-12 monoclonal antibody reacts with human Interferon Regulatory Factor (IRF)-3. IRF-3 is a 427 amino acid constitutively expressed member of the IRF transcription factor family that plays a critical role in the induction of type I interferons. Inactive IRF-3 continuously transits between the cytoplasm and nucleus. Virus or dsRNA induces the C-terminal phosphorylation of IRF-3 by IKKε and TBK-1. This phosphorylation results in retention of IRF-3 in the nucleus and subsequent transcription of IFN-β, several interferon stimulated genes (ISGs), and other antiviral response genes.

Applications Reported: This SL-12 (SL12) antibody has been reported for use in immunoblotting (WB).

Applications Tested: This SL-12 (SL12) antibody has been tested by immunoblot of normal human peripheral blood cell lysates. This antibody can be used at less than or equal to 2 µg/mL in blocking buffer. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

IFR3 (interferon regulatory factor 3) is a key transcriptional regulator of type I interferon (IFN)-dependent immune responses. It plays a critical role in the innate immune response against DNA and RNA viruses. IFR3 regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. It acts as a more potent activator of the IFN-beta gene than the IFN-alpha gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. IFR3 is found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA, or toll-like receptor signaling. It is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Mutations in the gene can result in encephalopathy, acute, infection-induced, herpes-specific 7.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Recruitment of activated IRF-3 and CBP/p300 to herpes simplex virus ICP0 nuclear foci: Potential role in blocking IFN-beta induction.

Virology

Melroe GT,Silva L,Schaffer PA,Knipe DM

Published figure using IRF3 monoclonal antibody (Product # 14-9947-82) in Western Blot

Tue Apr 10 00:00:00 UTC 2007

生物信息学

蛋白别名: Interferon regulatory factor 3; IRF-3; IRF3 (phospho S386); IRF3 (pS386); IRF3 (pSer386); IRF3 phospho; IRF3 phospho S386; IRF3 phospho Ser386; phospho IRF3

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基因别名: IIAE7; IRF3

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UniProt ID: (Human) Q14653

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Entrez Gene ID: (Human) 3661

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功能

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription cofactor activity protein binding identical protein binding protein homodimerization activity winged helix/forkhead transcription factor

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