Invitrogen

IRF7 Recombinant Rabbit Monoclonal Antibody (1H31L43)

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IRF7 Antibody (700229) in WB

Western blot was performed using Anti-IRF7 Recombinant Rabbit Monoclonal Antibody (1H31L43) (Product # 700229) and a 55kDa band corresponding to Interferon regulatory factor 7 was observed across cell lines. Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), Raji (Lane 2), HEK-293 (Lane 3), HEK-293 (IFN-a treated (500 units/mL for 12 hrs )(Lane 4), HeLa (Lane 5), HeLa (IFN-a treated (500 units/mL for 12 hrs) (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferr... View More

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IRF7 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using IRF7 Recombinant Rabbit Monoclonal Antibody (1H31L43) (Product # 700229), shows Upregulation upon treatment with IFN alpha, 500 units/mL for 24hrs in HEK293 and HeLa. {TM}

产品信息

700229

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:250-1:1000

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

1H31L43

抗原

A recombinant protein corresponding to amino acids 269-378.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2532301

产品详细信息

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Interferons (IFNs) are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-a/b genes. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. IRF7 play a major role in the innate immune pathway, interacting with the Toll-like receptor (TLR) adaptor proteins MyD88 and Tirp/TRAM and functioning as an intermediate TLR4 and TLR9 signaling. There are at least four differentially spliced isoforms of IRF7, although their function has not been clearly established.

仅用于科研。不用于诊断过程。未经明确授权不得转售。