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IRF8 Antibody (PA5-78013) in WB
Western blot was performed using Anti-IRF8 Polyclonal Antibody(Product # PA5-78013) and a 50kDa band corresponding to IRF8 was observed across cell lines tested except K-562 and T98G which are reported to be negative. A non-specific band around 95kDa was also observed across cell lines. Nuclear enriched extracts (30 µg lysate) of THP-1 (Lane 1), Raji (Lane 2), Ramos (Lane 3), K-562 (Lane 4) and T98G (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a N... View More
产品信息
PA5-78013
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein encompassing a sequence within the C-terminus region of human IRF8. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
0.45 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 1% BSA, 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2735964
产品详细信息
Positive Control: THP-1 nuclear extract
Predicted Reactivity: Mouse (86%), Rat (85%), Pig (84%), Rhesus Monkey (98%), Bovine (86%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
IRF8 (interferon regulatory factor 8) is a transcriptional activator or repressor. It specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes. IRF8 plays a negative regulatory role in cells of the immune system. It is involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells. This leads to recognition of AICE sequence, an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of the genes. Mutations in the gene can result in immunodeficiencey 32A and 32B.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: H-ICSBP; interferon consensus sequence binding protein 1; Interferon consensus sequence-binding protein; Interferon regulatory factor 8; IRF-8
基因别名: H-ICSBP; ICSBP; ICSBP1; IMD32A; IMD32B; IRF-8; IRF8
UniProt ID: (Human) Q02556
Entrez Gene ID: (Human) 3394
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
phagocytosis
immune response
myeloid cell differentiation
positive regulation of interferon-gamma production
positive regulation of interleukin-12 production
defense response to bacterium
defense response to protozoan
negative regulation of growth of symbiont in host
positive regulation of transcription initiation from RNA polymerase II promoter
interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
cellular response to lipopolysaccharide
