Invitrogen

IRF8 Monoclonal Antibody (ZI003)

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IRF8 Antibody (39-8800) in WB

Western blot analysis was performed on nuclear enriched extracts (30 µg lysate) of Raji (Lane 1), Ramos (Lane 2), K-562 (Lane 3), HL-60 (Lane 4), U-2 OS (Lane 5),THP-1 (Lane 6), NIH/3T3 (Lane 7) and T98G (Lane 8). The blot was probed with Anti- IRF8 Mouse Monoclonal Antibody (Product # 39-8800, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 48 kDa band corresponding to IRF8 was observed in Raji, Ramos and not observ... View More

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IRF8 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Relative expression of IRF8 was observed in Raji, Ramos, K-562, HL-60, U-2 OS, THP-1, NIH/3T3 and T98G in Western Blot using IRF8 Monoclonal Antibody (Product # 39-8800). IRF8 is reported to be expressed in B lymphocytes (Raji, Ramos) and not in other cell lines like K-562, HL-60, U-2 OS, THP-1, NIH/3T3 and T98G. {RE}

产品信息

39-8800

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

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酶联免疫吸附实验 (ELISA)

Assay-dependent

产品规格

种属反应

Human, Mouse

已发表种属

Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

ZI003

抗原

Synthetic peptide derived from the C-terminal region of the human ICSBP protein

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533439

靶标信息

IRF8 (interferon regulatory factor 8) is a transcriptional activator or repressor. It specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes. IRF8 plays a negative regulatory role in cells of the immune system. It is involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells. This leads to recognition of AICE sequence, an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of the genes. Mutations in the gene can result in immunodeficiencey 32A and 32B.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Decreased bone resorption in Ezh2 myeloid cell conditional knockout mouse model.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Caviness PC,Gai D,Lazarenko OP,Blackburn ML,Zhan F,Chen JR

Published figure using IRF8 monoclonal antibody (Product # 39-8800) in Western Blot

Sat Jul 01 00:00:00 UTC 2023

Monocyte Subsets With High Osteoclastogenic Potential and Their Epigenetic Regulation Orchestrated by IRF8.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Das A,Wang X,Kang J,Coulter A,Shetty AC,Bachu M,Brooks SR,Dell'Orso S,Foster BL,Fan X,Ozato K,Somerman MJ,Thumbigere-Math V

39-8800 was used in Western Blot to provide novel insights into the transcription factors and active cis-regulatory elements that regulate OC differentiation.

Fri Jan 01 00:00:00 UTC 2021

Identification of IRF8 as a potent tumor suppressor in murine acute promyelocytic leukemia.

Blood advances

Gaillard C,Surianarayanan S,Bentley T,Warr MR,Fitch B,Geng H,Passegué E,de Thé H,Kogan SC

39-8800 was used in Western Blotting to study the role of PML/PARA fusion protein in the initiation and maintenance of leukemogenesis.

Tue Oct 09 00:00:00 UTC 2018

生物信息学

蛋白别名: H-ICSBP; ICSBP; interferon consensus sequence binding protein 1; Interferon consensus sequence-binding protein; Interferon regulatory factor 8; IRF-8

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基因别名: AI893568; H-ICSBP; ICSBP; ICSBP1; IMD32A; IMD32B; IRF-8; IRF8; Myls

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UniProt ID: (Human) Q02556, (Mouse) P23611

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Entrez Gene ID: (Human) 3394, (Mouse) 15900

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功能

regulatory region DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding winged helix/forkhead transcription factor

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