Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 4
IRG1 Antibody (PA5-102893) in ICC/IF
Immunofluorescent analysis of IRG1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and IRG1 polyclonal antibody (Product # PA5-102893) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
产品信息
PA5-102893
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Not Applicable
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human ACOD1(Accession A6NK06), corresponding to amino acid residues I439-S481.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2815944
产品详细信息
Antibody detects endogenous levels of total IRG1.
靶标信息
Involved in the inhibition of the inflammatory response. Acts as a negative regulator of the Toll-like receptors (TLRs)- mediated inflammatory innate response by stimulating the tumor necrosis factor alpha-induced protein TNFAIP3 expression via reactive oxygen species (ROS) in LPS-tolerized macrophages. Involved in antimicrobial response of innate immune cells; IRG1- mediated itaconic acid production contributes to the antimicrobial activity of macrophages. Plays a role in the embryo implantation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Aconitate decarboxylase; Aconitate decarboxylase 1; CAD; Cis-aconitate decarboxylase; Cis-aconitic acid decarboxylase; Immune-responsive gene 1 protein; immune-responsive gene 1 protein homolog; immunoresponsive 1 homolog
基因别名: ACOD1; CAD; IRG1
UniProt ID: (Human) A6NK06
Entrez Gene ID: (Human) 730249
positive regulation of antimicrobial humoral response
defense response
inflammatory response
embryo implantation
metabolic process
negative regulation of NF-kappaB transcription factor activity
negative regulation of type I interferon production
negative regulation of toll-like receptor 2 signaling pathway
negative regulation of toll-like receptor 4 signaling pathway
cellular response to interferon-beta
negative regulation of innate immune response
negative regulation of inflammatory response
cellular response to molecule of bacterial origin
cellular response to lipopolysaccharide
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to progesterone stimulus
tolerance induction to lipopolysaccharide
positive regulation of reactive oxygen species metabolic process
