Invitrogen

ISCA1 Polyclonal Antibody

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抗体检测数据 (2)
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ISCA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ISCA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ISCA1 Antibody (PA5-60121) in IHC (P)

Immunohistochemical analysis of ISCA1 in human liver using ISCA1 Polyclonal Antibody (Product # PA5-60121) shows strong cytoplasmic positivity in hepatocytes.

ISCA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ISCA1 Antibody in Western Blot (WB)

ISCA1 Antibody in Western Blot, Immunohistochemistry (Paraffin) (WB, IHC (P))

ISCA1 Antibody in Western Blot (WB)

Product Image Gallery

ISCA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of ISCA1 in human liver using ISCA1 Polyclonal Antibody (Product # PA5-60121) shows strong cytoplasmic positivity in hepatocytes.

ISCA1 Antibody in Western Blot (WB) — Western blot analysis of ISCA1 in control (vector only transfected HEK293T lysate) and ISCA1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a ISCA1 Polyclonal Antibody (Product # PA5-60121).

ISCA1 Antibody in Western Blot, Immunohistochemistry (Paraffin) (WB, IHC (P)) — 1 FIGURE Generation of neuron-specific Isca1 knockout rats. (A) The expression of ISCA1 in a brain section from a WT rat detected by immunofluorescence staining. DAPI (blue) stained the nucleus, and TRITC (red) conjugated secondary antibody was used to detect the ISCA1-specific antibodies. Scale bar: 1000 mum. (B) PCR was used for the genotyping of Isca1 CKO rats. Among the 12 littermates, numbers 2 and 6 are homozygote, 5 and 9 are wild types, and 3 is heterozygote. (C) Schematic diagram describing the strategy for generating CKO rats. (D) Western blots using an ISCA1-specific antibody showed the absence of ISCA1 protein in the brain tissues of CKO rats. GAPDH was used as a loading control. (E) Representative images showed the absence of ISCA1 protein in different regions of WT and CKO rats by IHC staining at the eighth week (S1, primary somatosensory cortex; DG, dentate gyrus). Scale bar for overview, 1000 mum; scale bar for magnified view; 50 mum. (F) Body weights compared between WT and CKO rats. (G) A representative graph shows the appearance of WT and CKO rats at the eighth week. (H) Survival analysis for WT and CKO rats; n = 17/group.

ISCA1 Antibody in Western Blot (WB) — FIGURE 1 The expression of ISCA1 under cardiomyopathy and establishment of myocardium specific Isca1 knockout heterozygote (HET) rat. (A) Phenotypic characteristics of clinical patients with Isca1 mutation. (B) ISCA1 expression in myocardium from ischemic and non-ischemic cardiomyopathy patients compared with normal donors ( p < .01, data from GSE1869 , n = 6 in normal group; n = 10 in ischemic group; n = 21 in non-ischemic group). (C) The HET ( Isca1 flox/+ /alpha-MHC-Cre ) rat was obtained by crossing Isca1 -floxed rats with alpha-MHC-Cre rats. Genotyping assays were performed using PCR (D) and Isca1 expression in the myocardium of HET rats was detected using western blot (E-F, n = 3 rats per group, * p < .05, vs. WT)

产品信息

PA5-60121

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

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免疫组化（石蜡） (IHC (P))

1:200-1:500

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产品规格

种属反应

Human

已发表种属

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human ISCA1. Recombinant protein control fragment (Product #RP-100140).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2642786

产品详细信息

Immunogen sequence: DKLSSEFVFN NPNIKGTCGC GESFNI

Highest antigen sequence identity to the following orthologs: Mouse - 92%, Rat - 96%.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A neuron-specific Isca1 knockout rat developments multiple mitochondrial dysfunction syndromes.

Animal models and experimental medicine

Sheng H,Lu D,Qi X,Ling Y,Li J,Zhang X,Dong W,Chen W,Gao S,Gao X,Zhang L,Zhang L

PA5-60121 was used in Western Blot to investigate its pathological features and neuronal death.

Sat Apr 01 00:00:00 UTC 2023

A neuron-specific Isca1 knockout rat developments multiple mitochondrial dysfunction syndromes.

Animal models and experimental medicine

Sheng H,Lu D,Qi X,Ling Y,Li J,Zhang X,Dong W,Chen W,Gao S,Gao X,Zhang L,Zhang L

PA5-60121 was used in Western Blot to investigate its pathological features and neuronal death.

Sat Apr 01 00:00:00 UTC 2023

A neuron-specific Isca1 knockout rat developments multiple mitochondrial dysfunction syndromes.

Animal models and experimental medicine

Sheng H,Lu D,Qi X,Ling Y,Li J,Zhang X,Dong W,Chen W,Gao S,Gao X,Zhang L,Zhang L

PA5-60121 was used in Western Blot to investigate its pathological features and neuronal death.

Sat Apr 01 00:00:00 UTC 2023

Novel rat model of multiple mitochondrial dysfunction syndromes (MMDS) complicated with cardiomyopathy.

Animal models and experimental medicine

Ling Y,Ma J,Qi X,Zhang X,Kong Q,Guan F,Dong W,Chen W,Gao S,Gao X,Pan S,Ma Y,Lu D,Zhang L

PA5-60121 was used in Western Blotting to establish a rat model of MMDS complicated with cardiomyopathy which can be applied to the study of the mechanism of energy metabolism in cardiovascular diseases, as well as research and development of drugs.

Wed Dec 01 00:00:00 UTC 2021

Novel rat model of multiple mitochondrial dysfunction syndromes (MMDS) complicated with cardiomyopathy.

Animal models and experimental medicine

Ling Y,Ma J,Qi X,Zhang X,Kong Q,Guan F,Dong W,Chen W,Gao S,Gao X,Pan S,Ma Y,Lu D,Zhang L

PA5-60121 was used in Western Blotting to establish a rat model of MMDS complicated with cardiomyopathy which can be applied to the study of the mechanism of energy metabolism in cardiovascular diseases, as well as research and development of drugs.

Wed Dec 01 00:00:00 UTC 2021

Expanding the phenotype of mitochondrial disease: Novel pathogenic variant in ISCA1 leading to instability of the iron-sulfur cluster in the protein.

Mitochondrion

Lebigot E,Hully M,Amazit L,Gaignard P,Michel T,Rio M,Lombès M,Thérond P,Boutron A,Golinelli-Cohen MP

PA5-60121 was used in Western Blotting to report a patient carrying a novel pathogenic variant p.(Tyr101Cys) in ISCA1 leading to MMDS type 5.

Fri May 01 00:00:00 UTC 2020

生物信息学

蛋白别名: HESB like domain containing 2; HESB-like domain-containing protein 2; hIscA; iron sulfur assembly protein IscA; Iron-sulfur assembly protein IscA; Iron-sulfur cluster assembly 1 homolog, mitochondrial

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基因别名: GK004; HBLD2; hIscA; ISA1; ISCA1

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UniProt ID: (Human) Q9BUE6

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Entrez Gene ID: (Human) 81689

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structural molecule activity ferrous iron binding 2 iron, 2 sulfur cluster binding 4 iron, 4 sulfur cluster binding chaperone

sulfur compound metabolic process iron-sulfur cluster assembly protein maturation by iron-sulfur cluster transfer