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ISG15 Antibody (14-5857-82) in WB
Immunoblot of unstimulated (lane 1) or LPS-stimulated (lane 2) RAW264.7 cell line lysates with 2 µg/mL of Anti-Mouse ISG15 Purified. ISG-15 was upregulated in the activated sample. Co-incubation with anti-actin antibody confirmed equal loading. ISG-15, a ubiquitin-like protein, is conjugated to many cellular proteins leading to the large molecular weight signal. Bands were visualized using Anti-Armenian Hamster IgG HRP and Anti-Mouse IgG, respectively.
产品信息
14-5857-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Rat
宿主/亚型
Armenian hamster
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
3C2
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_1272273
产品详细信息
Description: This 3C2 monoclonal antibody reacts with mouse interferon stimulated gene-15 (ISG-15), a 15kDa ubiquitin-like protein (UBL) and cytokine. ISG-15 is translated as a precursor that is subsequently activated by an E1 enzyme, conjugated by an E2 enzyme via the C-terminal LRLRGG motif and ligated by an E3 ligase. The activity of ISG-15 is dependent on its modification of target proteins at specific lysine residues. Several viruses utilize ISG-15 and ubiquitin deconjugating proteases, while influenza B protein NS1 has been shown to specifically bind ISG-15 thereby blocking its conjugation.
In addition to its role as a protein modification, ISG-15 is secreted by monocytes and lymphocytes in response to IFNalpha, beta, or gamma, as well as influenza B virus. In this role as cytokine, it has been shown to induce secretion of IFNgamma and support NK cell proliferation and cytolytic activity.
Applications Reported: This 3C2 antibody has been reported for use in immunoblotting (WB).
Applications Tested: This 3C2 antibody has been tested by immunoblot of Raw 264.7 cell lysates at 2 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Ubiquitin-like protein ISG15 plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. It is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and an augmentation of non-MCH (major histocompatibility complex)-restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega, but not IFN-gamma. ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog. Mutations affecting the gene can result in Immunodeficiency 38, with basal ganglia calcification (IMD38).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: interferon, alpha-inducible protein; Interferon-induced 15 kDa protein; interferon-induced 15-KDa protein; Interferon-induced 17 kDa protein; interferon-stimulated protein (15 kDa); interferon-stimulated protein 15; IP17; Ubiquitin cross-reactive protein; Ubiquitin-like protein ISG15
基因别名: G1p2; IGI15; IP17; Irfp; Isg15; UCRP
UniProt ID: (Mouse) Q64339
Entrez Gene ID: (Mouse) 100038882
modification-dependent protein catabolic process
negative regulation of protein ubiquitination
ISG15-protein conjugation
regulation of interferon-gamma production
response to type I interferon
defense response to bacterium
negative regulation of viral genome replication
positive regulation of erythrocyte differentiation
defense response to virus
