Invitrogen

ISG20 Polyclonal Antibody

ISG20 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ISG20 Antibody (PA5-30073) in IHC (P)

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using ISG20 (Product # PA5-30073) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

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ISG20 Antibody — FIG 6 Generation of ISG20-resistant BUNV and demonstration that endogenous ISG20 possesses antibunyaviral activity. (A and B) Plaque phenotypes (on Vero and Vero-ISG20 cells) (A) and fold inhibition (B) of the parental virus and the P20 and P40 ISG20-resistant viruses by ISG20 (fold reductions in PFU per milliliter for comparisons of naive and Vero-ISG20 cells). (C) Comparison of growth curves for the parental virus and the P40 virus on Vero-ISG20 and naive Vero cells. Cells were infected at an MOI of 0.01 (data are means +- SD; n = 3). (D) Infectious yields (36 h after infection at an MOI of 0.01) of wild-type (wt) BUNV and reassortant mutants (mutant segments are denoted by "'") from Vero-ISG20 or Vero-D94G cells (data are means and ranges; n = 2). (E) Biological IFN assays to assess the ability of supernatants from HeLa cells infected with the indicated viruses to inhibit the replication of EMCV in A549-Npro cells. (F) Western blot analysis of basal and inducible expression in naive HeLa cells, HeLa-shGFP cells, and HeLa-shISG20 cells. Basal expression or universal interferon (500 U/mL)-induced ISG20 expression was stably detected in naive HeLa cells and HeLa-shGFP cells, whereas both basal and inducible ISG20 expression was undetectable in HeLa-shISG20 cells. (G) Growth curves for parental BUNV, P40 Vero-EMPTY-passaged BUNV, and P40 ISG20-resistant BUNV on HeLa cells (MOI = 0.01) (data are means +- SD; n = 3). (H) Growth curves for parental BUNV and P40 ISG20-resistant {TM}

产品信息

PA5-30073

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 1 and 181 of Human ISG20

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2547547

产品详细信息

Recommended positive controls: Raji, mouse spleen.

Predicted reactivity: Mouse (83%), Rat (82%), Pig (81%), Rhesus Monkey (97%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Interferon-induced antiviral exoribonuclease that acts on single-stranded RNA and also has minor activity towards single-stranded DNA. Exhibits antiviral activity against RNA viruses including hepatitis C virus (HCV), hepatitis A virus (HAV) and yellow fever virus (YFV) in an exonuclease-dependent manner. May also play additional roles in the maturation of snRNAs and rRNAs, and in ribosome biogenesis. [UniProt]

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

USP22 controls type III interferon signaling and SARS-CoV-2 infection through activation of STING.

Cell death & disease

Karlowitz R,Stanifer ML,Roedig J,Andrieux G,Bojkova D,Bechtel M,Smith S,Kowald L,Schubert R,Boerries M,Cinatl J,Boulant S,van Wijk SJL

PA5-30073 was used in Western Blotting to reveal USP22 as central host regulator of STING and type III IFN signaling, with important implications for SARS-CoV-2 infection and antiviral defense.

Sat Aug 06 00:00:00 UTC 2022

USP22 controls type III interferon signaling and SARS-CoV-2 infection through activation of STING.

Cell death & disease

Karlowitz R,Stanifer ML,Roedig J,Andrieux G,Bojkova D,Bechtel M,Smith S,Kowald L,Schubert R,Boerries M,Cinatl J,Boulant S,van Wijk SJL

PA5-30073 was used in Western Blotting to reveal USP22 as central host regulator of STING and type III IFN signaling, with important implications for SARS-CoV-2 infection and antiviral defense.

Sat Aug 06 00:00:00 UTC 2022

ISG15-dependent activation of the sensor MDA5 is antagonized by the SARS-CoV-2 papain-like protease to evade host innate immunity.

Nature microbiology

Liu G,Lee JH,Parker ZM,Acharya D,Chiang JJ,van Gent M,Riedl W,Davis-Gardner ME,Wies E,Chiang C,Gack MU

PA5-30073 was used in Western Blot to investigate the role of interferon (IFN)-stimulated gene 15 in innate immunity.

Thu Apr 01 00:00:00 UTC 2021

ISG15-dependent Activation of the RNA Sensor MDA5 and its Antagonism by the SARS-CoV-2 papain-like protease.

bioRxiv : the preprint server for biology

Liu G,Lee JH,Parker ZM,Acharya D,Chiang JJ,van Gent M,Riedl W,Davis-Gardner ME,Wies E,Chiang C,Gack MU

PA5-30073 was used in Western Blotting to demonstrate interferon-stimulated gene ISG15 is crucial for the ability of viral RNA sensor MDA5 to perform an antiviral response.

Tue Oct 27 00:00:00 UTC 2020

Interferon-Stimulated Gene (ISG)-Expression Screening Reveals the Specific Antibunyaviral Activity of ISG20.

Journal of virology

Feng J,Wickenhagen A,Turnbull ML,Rezelj VV,Kreher F,Tilston-Lunel NL,Slack GS,Brennan B,Koudriakova E,Shaw AE,Rihn SJ,Rice CM,Bieniasz PD,Elliott RM,Shi X,Wilson SJ

Published figure using ISG20 polyclonal antibody (Product # PA5-30073) in Western Blot

Sun Jul 01 00:00:00 UTC 2018

生物信息学

蛋白别名: Estrogen-regulated transcript 45 protein; interferon stimulated exonuclease; interferon stimulated exonuclease gene 20kDa; Interferon-stimulated gene 20 kDa protein; Promyelocytic leukemia nuclear body-associated protein ISG20; Protein DnaQL

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基因别名: 1600023I01Rik; 2010107M23Rik; 20kDa; CD25; DnaQL; HEM45; ISG20

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UniProt ID: (Human) Q96AZ6, (Mouse) Q9JL16

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Entrez Gene ID: (Human) 3669, (Mouse) 57444

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功能