Invitrogen

ISL2 Polyclonal Antibody

ISL2 Antibody in Immunocytochemistry (ICC/IF)

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ISL2 Antibody (PA5-80743) in ICC/IF

Immunofluorescence staining of ISL2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with ISL2 Polyclonal Antibody (Product # PA5-80743, 1:5,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus.

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产品信息

PA5-80743

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫细胞化学 (ICC/IF)

1:1,000-1:5,000

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to the center region of the Human ISL2

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

5 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2788025

产品详细信息

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Human ISL2.

靶标信息

ISL2 is a transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways.

仅用于科研。不用于诊断过程。未经明确授权不得转售。