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IST1 Antibody (PA5-97212) in WB
Western blot analysis of IST1 in extracts of various cell lines. Samples were incubated with IST1 Polyclonal antibody (Product # PA5-97212) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
PA5-97212
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human IST1 (NP_0012579061)
偶联物
形式
Liquid
浓度
1.87 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2809014
产品详细信息
Immunogen sequence: MLGSGFKAER LRVNLRLVIN RLKLLEKKKT ELAQKARKEI ADYLAAGKDE RARIRVEHII REDYLVEAME ILELYCDLLL ARFGLIQSMK ELDSGLAESV STLIWAAPRL QSEVAELKIV ADQLCAKYSK EYGKLCRTNQ IGTVNDRLMH KLSVEAPPKI LVERYLIEIA KNYNVPYEPD SVVMAEAPPG VETDLIDVGF TDDVKKGGPG RGGSGGFTAP VGGPDGTVPM PMPMPMPMPS ANTPFSYPLP KGPVDDINAD KNISSAQIVG PGPKPEASAK LPSRPADNYD NFVLPELPSV PDTLPTASAG ASTSASEDID FDDLSRRFEE LKKKT; Positive Samples: A-549, HeLa, Mouse heart, Rat brain; Cellular Location: Cytoplasm, Cytoplasmic vesicle, Midbody, centrosome, cytoskeleton, microtubule organizing center
靶标信息
Proposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Charged multivesicular body protein 8; CHMP8; hIST1; increased sodium tolerance 1 homolog; IST1 homolog; IST1, endosomal sorting complex required for transport-III component; MAPK activating protein PM28; MGC117220; overexpressed in lung cancer 1; pm28; Putative MAPK-activating protein PM28
基因别名: 2400003C14Rik; AW536298; IST1; KIAA0174; mKIAA0174; OLC1; RGD1307799
UniProt ID: (Human) P53990, (Rat) Q568Z6, (Mouse) Q9CX00
Entrez Gene ID: (Human) 9798, (Rat) 307833, (Mouse) 71955
cytokinesis
positive regulation of defense response to virus by host
protein localization
abscission
protein transport
viral release from host cell
multivesicular body assembly
establishment of protein localization
positive regulation of proteolysis
viral capsid secondary envelopment
positive regulation of collateral sprouting
cell division
cell-cell adhesion
mitophagy in response to mitochondrial depolarization
xenophagy
ESCRT III complex disassembly
cell cycle
