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ITGA4 Antibody (MA1-80529) in ICC/IF
Immunofluorescence analysis of ITGA4 was performed using 70% confluent log phase MOLT-4 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ITGA4 Mouse Monoclonal Antibody (HP2/1) (Product # MA1-80529) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at... View More
产品信息
MA1-80529
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Many,
Mouse,
Rat
已发表种属
Not Applicable
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
HP2/1
抗原
JM leukemia line.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
purified
保存液
PBS
内含物
0.1% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_928544
产品详细信息
Clone HP2/1 binds to both intact and the 80kDa fragment of integrin alpha-4. This antibody can be used in basic studies of VLA-4 mediated adhesion and its interaction with the VCAM-1 structure and has been demonstrated to inhibit cell binding to soluble VCAM-1.
This product contains sodium azide; removal by dialysis is recommended prior to use in functional assays.
Mouse anti Human CD49d monoclonal antibody, clone HP2/1 recognizes human CD49d also known as integrin alpha-4 or VLA-4 subunit alpha.
靶标信息
The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 269C wild type; alpha 4 subunit of VLA-4 receptor; antigen CD49D, alpha-4 subunit of VLA-4 receptor; CD49 antigen-like family member D; CD49d; Integrin alpha-4; Integrin alpha-IV; integrin alpha4B; ITGA-IV; LPAM subunit alpha; Lymphocyte Peyer patch adhesion molecules subunit alpha; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 receptor, alpha 4 subunit; VLA-4 subunit alpha
基因别名: CD49D; IA4; ITGA4; Itga4B
UniProt ID: (Human) P13612, (Mouse) Q00651
Entrez Gene ID: (Human) 3676, (Rat) 311144, (Mouse) 16401
blood vessel remodeling
positive regulation of leukocyte migration
cell-matrix adhesion involved in ameboidal cell migration
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
heart development
cell migration
heterotypic cell-cell adhesion
substrate adhesion-dependent cell spreading
endodermal cell differentiation
receptor clustering
leukocyte tethering or rolling
diapedesis
face development
chorio-allantoic fusion
cellular response to cytokine stimulus
T cell migration
negative regulation of protein homodimerization activity
import into cell
positive regulation of leukocyte cell-cell adhesion
positive regulation of leukocyte tethering or rolling
positive regulation of T cell migration
single organismal cell-cell adhesion
mesenchyme development
visceral serous pericardium development
B cell differentiation
extracellular matrix organization
regulation of immune response
leukocyte migration
clathrin-mediated extracellular exosome endocytosis
