Invitrogen

ITK Polyclonal Antibody

ITK Antibody in Immunocytochemistry (ICC/IF)

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ITK Antibody (PA5-29669) in ICC/IF

Immunofluorescence analysis of Tyrosine-protein kinase ITK/TSK was performed using Jurkat and Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ITK Polyclonal Antibody (Product # PA5-29669) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A3... View More

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ITK Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-ITK Polyclonal Antibody (Product # PA5-29669) shows cytoplasmic and membrane staining for ITK in Jurkat cells but not in Raji. ITK expression is reported to be seen in T cells (Jurkat) and natural killer cells but not in B cells (Raji). {RE}

产品信息

PA5-29669

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 117 and 346 of Human ITK

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.31 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2547144

产品详细信息

Recommended positive controls: Molt-4, Jurkat.

Predicted reactivity: Mouse (92%), Rat (93%), Rhesus Monkey (98%), Bovine (94%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

ITK is a tyrosine kinase expressed in T-cells which contains both SH2 and SH3 domains. ITK is thought to play a role in T-cell proliferation and differentiation. The Tec family of non-receptor tyrosine kinases is composed of six proteins designated Tec, Itk (also known as Emt or Tsk), Btk (previously known as Atk, BPK or Emb), Bmx, Txk (also known as Rlk) and Dsrc28C. All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain pleckstrin homology (PH) and Tec homology (TH) domains in their amino termini. Itk associates with CD28 and becomes activated subsequent to CD28 ligation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: homolog of mouse T-cell itk/tsk; IL-2-inducible T cell kinase; IL-2-inducible T-cell kinase; IL2-inducible T-cell kinase; interleukin-2-inducible T cell kinase; Interleukin-2-inducible T-cell kinase; Kinase EMT; Kinase TLK; MGC126257; MGC126258; T-cell-specific kinase; Tyrosine-protein kinase ITK/TSK; Tyrosine-protein kinase Lyk

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基因别名: EMT; ITK; LPFS1; LYK; PSCTK2; Tcsk; Tlk; Tsk

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UniProt ID: (Human) Q08881, (Mouse) Q03526

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Entrez Gene ID: (Human) 3702, (Mouse) 16428

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