Invitrogen

ITK Monoclonal Antibody (2F12), eBioscience™

ITK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ITK Antibody (14-9752-82) in IHC (P)

Immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue using 1 µg/mL of Mouse IgG1 K Isotype Control Purified (left) or 1 µg/mL of Anti-ITK Purified (right) followed by Anti-Mouse IgG Biotin, Streptavidin-HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.

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ITK Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines and tissues tested owing to their inherent genetic constitution. Relative expression of Tyrosine-protein kinase ITK/TSK was observed in T cell lines (Jurkat and MOLT-4) in comparison to B cell lines (Raji and Ramos) and in mouse thymus in comparison to skeletal muscle and heart using Anti-ITK Monoclonal Antibody (2F12), eBioscience™ (Product # 14-9752-82) in Western Blot. {RE}

产品信息

14-9752-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

2F12

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_2572984

产品详细信息

Description: The monoclonal antibody 2F12 recognizes human, mouse, and rat ITK. ITK (also known as Interleukin-2 inducible T-cell kinase, T-cell-specific kinase, Tyrosine-protein kinase Lyk, Emt, and Tsk) is a member of the Tec family of kinases that include Tec, Btk, Bmx, and Txk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. ITK is highly expressed by T cells and is required for optimal TCR-induced PLCgamma1 activation. Mice congenitally lacking ITK show profound defects in T cell development and signaling. Defects in ITK are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1). ITK also plays a role in the development of various T cell mediated inflammatory disorders such as atopic dermatitis, psoriasis, and allergic asthma.

Applications Reported: This 2F12 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy.

Applications Tested: This 2F12 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

ITK is a tyrosine kinase expressed in T-cells which contains both SH2 and SH3 domains. ITK is thought to play a role in T-cell proliferation and differentiation. The Tec family of non-receptor tyrosine kinases is composed of six proteins designated Tec, Itk (also known as Emt or Tsk), Btk (previously known as Atk, BPK or Emb), Bmx, Txk (also known as Rlk) and Dsrc28C. All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain pleckstrin homology (PH) and Tec homology (TH) domains in their amino termini. Itk associates with CD28 and becomes activated subsequent to CD28 ligation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: homolog of mouse T-cell itk/tsk; IL-2-inducible T cell kinase; IL-2-inducible T-cell kinase; IL2-inducible T-cell kinase; interleukin-2-inducible T cell kinase; Interleukin-2-inducible T-cell kinase; Kinase EMT; Kinase TLK; MGC126257; MGC126258; T-cell-specific kinase; Tyrosine-protein kinase ITK/TSK; Tyrosine-protein kinase Lyk

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基因别名: EMT; ITK; LPFS1; LYK; PSCTK2; Tcsk; Tlk; Tsk

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UniProt ID: (Human) Q08881, (Mouse) Q03526

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Entrez Gene ID: (Human) 3702, (Rat) 363577, (Mouse) 16428

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