Invitrogen

IYD Polyclonal Antibody

IYD Antibody in Immunocytochemistry (ICC/IF)

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IYD Antibody (PA5-100584) in ICC/IF

Immunocytochemistry analysis of IYD in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with IYD polyclonal antibody (Product # PA5-100584) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

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产品信息

PA5-100584

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human IYD(Accession Q6PHW0), corresponding to amino acid residues R104-E154.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2850092

产品详细信息

Antibody detects endogenous levels of total IYD.

靶标信息

This gene encodes an enzyme that catalyzes the oxidative NADPH-dependent deiodination of mono- and diiodotyrosine, which are the halogenated byproducts of thyroid hormone production. The N-terminus of the protein functions as a membrane anchor. Mutations in this gene cause congenital hypothyroidism due to dyshormonogenesis type 4, which is also referred to as deiodinase deficiency, or iodotyrosine dehalogenase deficiency, or thyroid hormonogenesis type 4. Alternative splicing results in multiple transcript variants.

仅用于科研。不用于诊断过程。未经明确授权不得转售。