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IgM Antibody (12-4342-82) in Flow
Staining of BALB/c splenocytes with Anti-Mouse CD49b (Integrin alpha 2) APC (cloen DX5, a rat IgM isotype) (Product # 17-5971-82) and subsequently stained with buffer (autofluorescence) (left) or Anti-Rat IgM PE (right). Total viable cells were used for analysis.
产品信息
12-4342-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Rat
已发表种属
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
RM-7B4
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10668833
产品详细信息
Description: The monoclonal antibody RM-7B4 recognizes Rat IgGM antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgM or any mouse isotype antibodies.
Applications Reported: This RM-7B4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This RM-7B4 antibody has been tested by flow cytometric analysis of cells stained with a primary rat IgM antibody. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Rat IgM is an immunoglobulin M isotype antibody produced by B cells in rats. It plays a crucial role in the immune system by recognizing and neutralizing antigens, such as bacteria and viruses. Rat IgM has been extensively studied and is widely used in research applications, including immunology, microbiology, and biotechnology. It is commonly used as a control in experiments, as well as a diagnostic tool for detecting infectious diseases and autoimmune disorders. Researchers have developed various techniques for producing and purifying Rat IgM, including hybridoma technology, protein A/G chromatography, and affinity chromatography. Rat IgM is a valuable tool in the field of immunology, and its use has contributed to a better understanding of the immune system and the development of new treatments for various diseases.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
基因别名: IgM; RGD1359202
Entrez Gene ID: (Rat) 299357
