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Integrin beta 5 Antibody (14-0497-82) in Flow
Staining of Chinese Hamster Ovary (CHO) cell line with 0.25 µg of Mouse IgG1kappa Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.25 µg of Anti-Integrin beta 5 Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Total viable cells were used for analysis.
产品信息
14-0497-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Hamster,
Human,
Mouse
已发表种属
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
KN52
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_467288
产品详细信息
Description: The KN52 antibody reacts with human integrin beta 5, an approximately 100,000 Mr (reduced) and 95,000 Mr (nonreduced) a member of the integrin family. Only a single alpha chain, the alpha v subunit, associates with the integrin beta 5 to form the vitronectin receptor complex. Integrin alpha v/beta 5 complex also binds to the basic domain of Tat (he sequence RKKRRQRRR). The integrin beta 5 is found on carcinoma cell lines, hepatoma and fibroblast cell lines, and is absent from lymphoblastoid cells and platelets.
The KN52 monoclonal antibody has been shown to inhibit migration.
Applications Reported: This KN52 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This KN52 antibody has been tested by flow cytometric analysis of Chinese Hamster Ovary (CHO), A549 and normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Integrins are important extracellular matrix (ECM) receptor proteins located on cell surfaces. They are hetrodimers composed of an alpha and a beta transmembrane glycoprotein subunit. Around 22 different integrins (different alpha/ beta subunit combinations) are found in nature. Integrins are generally present in high concentrations at the cell surface, but, unlike most other cell-surface receptors, they bind ligands with very low affinity. Due to their weak individual binding, integrins need to cluster and bind in-groups in order to effectively bind the ECM. Integrins bind many different ligands including laminin. Each integrin is made up of a large N-terminal extracellular domain that binds the ECM ligand and a small C-terminal cytoplasmic domain that mediates interaction with the actin cytoskeleton and signaling function.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD61; FLJ26658; GP3A; GPIIIa; integrin beta 5; Integrin beta-5; integrin, beta 5; testis secretory sperm-binding protein Li 217p
基因别名: [b]-5; [b]5; [b]5A; [b]5B; AA475909; AI874634; beta-5; beta5; ESTM23; I79_000655; ITGB5
UniProt ID: (Human) P18084, (Mouse) O70309
Entrez Gene ID: (Human) 3693, (Mouse) 16419, (Chinese hamster) 100765378
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
muscle contraction
cell-matrix adhesion
transforming growth factor beta receptor signaling pathway
integrin-mediated signaling pathway
extracellular matrix organization
endodermal cell differentiation
stress fiber assembly
viral entry into host cell
epithelial cell-cell adhesion
cell adhesion
