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Integrin beta 7 Antibody (13-5867-82) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0038-42) and 0.125 µg of Rat IgG2a K Isotype Control Biotin (Product # 13-4321-82) (left) or 0.06 µg of Anti-Human/Mouse Integrin beta 7 Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.
产品信息
13-5867-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Not Applicable
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
FIB504
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_1518756
产品详细信息
Description: The FIB504 monoclonal antibody reacts with human and mouse integrin beta7. Integrin beta7 is a 130 kD glycoprotein which associates with integrin alpha4 (CD49d) to form the alpha4beta7 integrin LPAM-1, expressed on intraepithelial lymphocytes. It also associates with alphaE (CD103) to form the alphaEbeta7 integrin HML-1, expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes. Main ligands for integrin alpha4beta7 include VCAM-1 (CD106), MAdCAM-1 and fibronectin, while the main ligand of integrin alphaEbeta7 is E-cadherin (CD324). Integrin beta7 plays an important role in the adhesion of leukocytes to endothelial cells promoting the transmigration of leukocytes to extravascular spaces during the inflammatory response. The FIB504 antibody has been reported to have blocking activity.
Applications Reported: This FIB504 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This FIB504 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Integrin beta 7 associates with integrin alpha 4 (CD49d) to form the alpha 4 beta 7 integrin LPAM-1, expressed on intraepithelial lymphocytes. It also associates with alpha E (CD103) to form the alpha E beta 7 integrin HML-1, expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes. Ligands for LPAM-1 include VCAM-1 (CD106), MAdCAM-1 and fibronectin, while the ligand of HML-1 is E-cadherin (CD324). Integrin beta 7 plays an important role in the adhesion of leukocytes to endothelial cells promoting the transmigration of leukocytes to extravascular spaces during the inflammatory response.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Gut homing receptor beta subunit; integrin beta 7 subunit; Integrin beta-7; Integrin beta-P; integrin, beta 7; M290 IEL antigen
基因别名: ITGB7; Ly69
UniProt ID: (Human) P26010, (Mouse) P26011
Entrez Gene ID: (Human) 3695, (Mouse) 16421
cell-matrix adhesion involved in ameboidal cell migration
cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
extracellular matrix organization
heterotypic cell-cell adhesion
substrate adhesion-dependent cell spreading
receptor clustering
viral entry into host cell
regulation of immune response
leukocyte tethering or rolling
T cell migration
leukocyte migration
