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Interferon gamma Antibody (201-401-C41) in WB
Western blot using Rocklands protein-A purified anti-bovine IFN gamma antibody shows detection of recombinant bovine IFN gamma at 16.9 kDa, raised in yeast. Primary antibody was diluted to 1µg/mL. 3% BSA from Rocklands BSA-30 (Bovine Serum Albumin Solution) was used for blocking. Secondary antibody 611-131-122 (Goat anti-Rabbit IgG IRDye 800) was used at 1:20,000.
产品信息
201-401-C41
产品规格
种属反应
Bovine
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast corresponding to the 143 amino acids of the mature Bovine IFN Gamma protein.
偶联物
形式
Lyophilized
浓度
1 mg/mL
纯化类型
Protein A
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is specific for bovine IFN gamma protein. A BLAST analysis was used to suggest cross-reactivity with IFN gamma from bovine based on 100% homology; cross-reactivity to yak, bison, zebu, buffalo, goat, sheep, nilgai, giraffe, Chinese forest musk deer, sika deer, red deer, Arabian camel, and Bactrian camel based on 91-99% homology with the immunizing sequence. Cross-reactivity with IFN gamma from other sources has not been determined.
靶标信息
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: BoIFNG; H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Interferon; Interferon gamma; interferon gamma type 2; Interferon y; Interferon γ; interferon-gamma precursor; Interferonγ; M-IFN-g; R-IFN-g
基因别名: IFNG
UniProt ID: (Bovine) P07353
Entrez Gene ID: (Bovine) 281237
microglial cell activation
macrophage activation involved in immune response
cell cycle arrest
positive regulation of autophagy
positive regulation of epithelial cell migration
regulation of protein ADP-ribosylation
macrophage differentiation
positive regulation of B cell proliferation
positive regulation of protein complex assembly
negative regulation of interleukin-17 production
positive regulation of interleukin-12 production
positive regulation of interleukin-23 production
positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation involved in immune response
positive regulation of peptidyl-serine phosphorylation of STAT protein
positive regulation of smooth muscle cell apoptotic process
regulation of growth
positive regulation of protein import into nucleus
positive regulation of tyrosine phosphorylation of STAT protein
positive regulation of chemokine biosynthetic process
positive regulation of nitric oxide biosynthetic process
positive regulation of osteoclast differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
astrocyte activation
negative regulation of smooth muscle cell proliferation
positive regulation of cytokine secretion
positive regulation of inflammatory response
positive regulation of neurogenesis
regulation of insulin secretion
positive regulation of membrane protein ectodomain proteolysis
defense response to virus
positive regulation of killing of cells of other organism
interferon-gamma-mediated signaling pathway
positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity
positive regulation of fructose 1,6-bisphosphate metabolic process
positive regulation of calcidiol 1-monooxygenase activity
positive regulation of protein serine/threonine kinase activity
positive regulation of protein deacetylation
extrinsic apoptotic signaling pathway
positive regulation of neuron death
positive regulation of beta-amyloid formation
negative regulation of tau-protein kinase activity
positive regulation of core promoter binding
positive regulation of tumor necrosis factor (ligand) superfamily member 11 production
