Invitrogen

Involucrin Polyclonal Antibody

Involucrin Antibody in Immunocytochemistry (ICC/IF)

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Involucrin Antibody (PA5-32454) in ICC/IF

Immunofluorescence analysis of Involucrin was performed using 70% confluent log phase HaCaT and MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 45 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Involucrin Polyclonal Antibody (Product # PA5-32454) at 1:100 in 0.1% BSA, incubated at 4 de... View More

Involucrin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Involucrin Antibody in Western Blot (WB)

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产品信息

PA5-32454

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100

免疫细胞化学 (ICC/IF)

1:100

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encoding aa 420-578 of human involucrin.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.267 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.6, with 1% BSA

内含物

0.1% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2549923

产品详细信息

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is skin tissue.

靶标信息

Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures it is expressed by all cells that have left the basal layer. Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions and is reduced in severe dysplasias of the larynx and cervix. Its gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Global mapping of GalNAc-T isoform-specificities and O-glycosylation site-occupancy in a tissue-forming human cell line.

Nature communications

Nielsen MI,de Haan N,Kightlinger W,Ye Z,Dabelsteen S,Li M,Jewett MC,Bagdonaite I,Vakhrushev SY,Wandall HH

PA1-37934 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to find over 300 unique glycosylation sites across a diverse set of proteins specifically regulated by one of the GalNAc-T isoforms, consistent with their impact on the tissue phenotypes.

Fri Oct 21 00:00:00 UTC 2022

Global mapping of GalNAc-T isoform-specificities and O-glycosylation site-occupancy in a tissue-forming human cell line.

Nature communications

Nielsen MI,de Haan N,Kightlinger W,Ye Z,Dabelsteen S,Li M,Jewett MC,Bagdonaite I,Vakhrushev SY,Wandall HH

Fri Oct 21 00:00:00 UTC 2022

Essential Functions of Glycans in Human Epithelia Dissected by a CRISPR-Cas9-Engineered Human Organotypic Skin Model.

Developmental cell

Dabelsteen S,Pallesen EMH,Marinova IN,Nielsen MI,Adamopoulou M,Rømer TB,Levann A,Andersen MM,Ye Z,Thein D,Bennett EP,Büll C,Moons SJ,Boltje T,Clausen H,Vakhrushev SY,Bagdonaite I,Wandall HH

PA1-37934 was used in Immunocytochemistry-immunoflourescence to generate a library of 3D organotypic skin tissues that selectively differ in their capacity to produce glycan structures on the main types of N- and O-linked glycoproteins and glycolipids.