Invitrogen

JAM3 Polyclonal Antibody

JAM3 Antibody in Immunocytochemistry (ICC/IF)

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JAM3 Antibody (40-9000) in ICC/IF

Immunofluorescence analysis of JAM-C was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with JAM-C Rabbit Polyclonal Antibody (Product # 40-9000) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product #... View More

JAM3 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

JAM3 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

JAM3 Antibody — JAM-C knockdown inhibits hRPE cell migration. (A) Real-time PCR shows that in hRPE cells, JAM-C expression is reduced to approximately 20% of normal level after JAM-C shRNA transfection. (B) Immunoblot analysis shows that JAM-C protein expression decreases after JAM-C shRNA transfection in hRPE cells. (C) JAM-C knockdown inhibited hRPE cell migration compared with vector-treated cells or nontreated cells when using conditioned medium from the macrophages derived as a chemoattractant from patients with wet AMD. Migrated RPE cells were shown as a percentage of the control (first column). There was no significant difference among the three groups when 10% FBS or 0.2% FBS medium was used as a chemoattractant (n = 3). *P < 0.01. {KD}

产品信息

40-9000

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

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免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

2-3 µg/mL

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酶联免疫吸附实验 (ELISA)

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide derived from an internal region of the mouse and rat JAM-C proteins, which differs from human, bovine, and dog by one conservative amino acid replacement

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533486

靶标信息

JAM3 is involved in tight junctions and represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Soluble JAM-C Ectodomain Serves as the Niche for Adipose-Derived Stromal/Stem Cells.

Biomedicines

Yamazaki M,Sugimoto K,Mabuchi Y,Yamashita R,Ichikawa-Tomikawa N,Kaneko T,Akazawa C,Hasegawa H,Imura T,Chiba H

40-9000 was used in Immunohistochemistry to investigate if junctional adhesion molecules exhibit a novel mode of interaction and biological activity in adipose-derived stromal/stem cells (ADSCs).

Wed Mar 10 00:00:00 UTC 2021

Soluble JAM-C Ectodomain Serves as the Niche for Adipose-Derived Stromal/Stem Cells.

Biomedicines

Yamazaki M,Sugimoto K,Mabuchi Y,Yamashita R,Ichikawa-Tomikawa N,Kaneko T,Akazawa C,Hasegawa H,Imura T,Chiba H

40-9000 was used in Immunohistochemistry to investigate if junctional adhesion molecules exhibit a novel mode of interaction and biological activity in adipose-derived stromal/stem cells (ADSCs).

Wed Mar 10 00:00:00 UTC 2021

Soluble JAM-C Ectodomain Serves as the Niche for Adipose-Derived Stromal/Stem Cells.

Biomedicines

Yamazaki M,Sugimoto K,Mabuchi Y,Yamashita R,Ichikawa-Tomikawa N,Kaneko T,Akazawa C,Hasegawa H,Imura T,Chiba H

40-9000 was used in Immunohistochemistry to investigate if junctional adhesion molecules exhibit a novel mode of interaction and biological activity in adipose-derived stromal/stem cells (ADSCs).

Wed Mar 10 00:00:00 UTC 2021

Targeting of junctional adhesion molecule-C inhibits experimental choroidal neovascularization.

Investigative ophthalmology & visual science

Hou X,Hu D,Wang YS,Tang ZS,Zhang F,Chavakis T,Li Y,Li X

Thu Mar 01 00:00:00 UTC 2012

Targeting of junctional adhesion molecule-C inhibits experimental choroidal neovascularization.

Investigative ophthalmology & visual science

Hou X,Hu D,Wang YS,Tang ZS,Zhang F,Chavakis T,Li Y,Li X

Thu Mar 01 00:00:00 UTC 2012

Spermatogonial stem cells alone are not sufficient to re-initiate spermatogenesis in the rat testis following adjudin-induced infertility.

International journal of andrology

Mok KW,Mruk DD,Lee WM,Cheng CY

Wed Feb 01 00:00:00 UTC 2012

Spermatogonial stem cells alone are not sufficient to re-initiate spermatogenesis in the rat testis following adjudin-induced infertility.

International journal of andrology

Mok KW,Mruk DD,Lee WM,Cheng CY

Wed Feb 01 00:00:00 UTC 2012

Evidence for cross-reactivity of JAM-C antibodies: implications for cellular localization studies.

Biology of the cell

Betanzos A,Schnoor M,Severson EA,Liang TW,Parkos CA

Thu Jun 04 00:00:00 UTC 2009

Evidence for cross-reactivity of JAM-C antibodies: implications for cellular localization studies.

Biology of the cell

Betanzos A,Schnoor M,Severson EA,Liang TW,Parkos CA

Thu Jun 04 00:00:00 UTC 2009

生物信息学

蛋白别名: JAM-2; JAM-3; JAM-C; junction cell adhesion molecule 3; junction cell adhesion molecule C; Junctional adhesion molecule 3; Junctional adhesion molecule C

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基因别名: 1110002N23Rik; JAM-2; JAM-3; JAM-C; JAM3; JAMC; Jcam3; UNQ859/PRO1868

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UniProt ID: (Human) Q9BX67, (Mouse) Q9D8B7

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Entrez Gene ID: (Human) 83700, (Mouse) 83964

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功能

integrin binding protein binding protein homodimerization activity protein heterodimerization activity

参与通路