Invitrogen

JMJD1B Recombinant Rabbit Monoclonal Antibody (1A5)

JMJD1B Antibody in Immunocytochemistry (ICC/IF)

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JMJD1B Antibody (MA5-44598) in ICC/IF

Immunofluorescence analysis of JMJD1B was performed using 70% confluent log phase HCT 116 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with JMJD1B Recombinant Rabbit Monoclonal Antibody (1A5) (Product # MA5-44598) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Al... View More

JMJD1B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

MA5-44598

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:400-1:2,000

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

1A5

抗原

Recombinant protein within human JMJD1B aa 251-500.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2931058

产品详细信息

Predicted band size: 191 kDa.

Positive Control: Hela cell lysate, NIH/3T3 cell lysate, A431, Hela, human testis tissue, human fallopian tube tissue.

Subcellular Location: Nucleus.

靶标信息

Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: [histone H3]-dimethyl-L-lysine(9) demethylase 3B; JmjC domain-containing histone demethylation protein 2B; jumonji domain containing 1B; Jumonji domain-containing protein 1B; lysine (K)-specific demethylase 3B; Lysine-specific demethylase 3B; Nuclear protein 5qNCA

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基因别名: 5830462I21Rik; 5qNCA; C5orf7; JHDM2B; JMJD1B; KDM3B; KIAA1082; mKIAA1082; NET22

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UniProt ID: (Human) Q7LBC6, (Mouse) Q6ZPY7

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Entrez Gene ID: (Human) 51780, (Mouse) 277250

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