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JMJD2B Antibody (A301-478A) in WB
Detection of human JMJD2B by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-JMJD2B antibody (Product # A301-478A) (lot A301-478A-2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A301-478A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1056 and 1096 of human jumonji domain containing 2B.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: [histone H3]-trimethyl-L-lysine(9) demethylase 4B; JmjC domain-containing histone demethylation protein 3B; jumonji domain containing 2B; Jumonji domain-containing protein 2B; lysine (K)-specific demethylase 4B; Lysine-specific demethylase 4B; tudor domain containing 14B
Gene Aliases: 4732474L06Rik; JHDM3B; JMJD2B; KDM4B; KIAA0876; mKIAA0876; TDRD14B
UniProt ID: (Human) O94953, (Mouse) Q91VY5
Entrez Gene ID: (Human) 23030, (Mouse) 193796
zinc ion binding
histone demethylase activity
dioxygenase activity
sulfonate dioxygenase activity
oxidoreductase activity
2,4-dichlorophenoxyacetate alpha-ketoglutarate dioxygenase activity
procollagen-proline dioxygenase activity
hypophosphite dioxygenase activity
DNA-N1-methyladenine dioxygenase activity
metal ion binding
C-19 gibberellin 2-beta-dioxygenase activity
C-20 gibberellin 2-beta-dioxygenase activity
histone modifying enzyme
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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