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Product Details
H00133746-M01
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
2D7
Immunogen
JMY (NP_689618, 535 a.a. approximately 634 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Product may be used with Western Blot (Cell lysate).
Immunogen sequence: SPMDEVLASL KRGSFHLKKV EQRTLPPFPD EDDSNNILAQ IRKGVKLKKV QKDVLRESFT LLPDTDPLTR SIHEALRRIK EASPESEDEE EALPCTDWEN
Target Information
JMY acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ37870; Junction-mediating and -regulatory protein; junction-mediating and regulatory protein; WAS protein homology region 2 domain containing 1-like 3
Gene Aliases: JMY; WHAMM2; WHDC1L3
UniProt ID: (Human) Q8N9B5
Entrez Gene ID: (Human) 133746
DNA repair
regulation of transcription from RNA polymerase II promoter
cell cycle arrest
Arp2/3 complex-mediated actin nucleation
positive regulation of apoptotic process
positive regulation of sequence-specific DNA binding transcription factor activity
'de novo' actin filament nucleation
actin polymerization-dependent cell motility
regulation of signal transduction by p53 class mediator
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