Invitrogen

JNK2 Polyclonal Antibody

JNK2 Antibody in Immunocytochemistry (ICC/IF)

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JNK2 Antibody (PA5-17634) in ICC/IF

Immunofluorescence analysis of JNK2 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with JNK2 Polyclonal Antibody (Product # PA5-17634) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 conjugate (Product # A27... View More

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JNK2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with JNK2 siRNA and a steep decrease in signal intensity was observed in western blot application using Anti-JNK2 Polyclonal Antibody (Product # PA5-17634). {KD}

产品信息

PA5-17634

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫细胞化学 (ICC/IF)

1:200

产品规格

种属反应

Hamster, Human, Mouse, Non-human primate, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to human JNK2

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

9 µg/mL

纯化类型

Antigen affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10979604

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

MAPK9 (JNK2) is a serine/threonine kinase which plays an important role in the MAPK pathway. JNK2 (p54a, SAPK1a), along with JNK1 and JNK3, is thought to play an important role in nuclear signal transduction through its environmental stress activation and subsequent phosphorylation of the nuclear transcription factor p53.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: c-Jun kinase 2; c-Jun N-terminal kinase 2; JNK; JNK-46; JNK-55; JNK/SAPK alpha; JNK1; JNK1A2; JNK21B1/2; Jun kinase; MAP kinase 10; MAP kinase 8; MAP kinase 9; MAPK 10; MAPK 9; MAPK10; MAPK8; mitogen activated protein kinase 9; Mitogen-activated protein kinase 9; OTTHUMP00000161542; OTTHUMP00000161543; p54-alpha; PRKM8; protein kinase, mitogen-activated 9; SAPK-alpha; SAPK1; SAPK1a; SAPK1c; stress activated protein kinase alpha II; Stress-activated protein kinase 1a; Stress-activated protein kinase JNK2

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基因别名: AI851083; I79_012561; JNK-55; JNK2; JNK2A; JNK2ALPHA; JNK2B; JNK2BETA; MAPK9; p54a; p54aSAPK; PRKM9; SAPK; SAPK1A

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UniProt ID: (Human) P45984, (Rat) P49186, (Mouse) Q9WTU6