Invitrogen

JNK2 Recombinant Rabbit Monoclonal Antibody (SC05-85)

JNK2 Antibody in Immunocytochemistry (ICC/IF)

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JNK2 Antibody (MA5-32191) in ICC/IF

Immunofluorescence analysis of JNK2 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with JNK2 Recombinant Rabbit Monoclonal Antibody (SC05-85) (Product # MA5-32191) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 ... View More

JNK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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JNK2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Western blot analysis using Anti-JNK2 Recombinant Rabbit Monoclonal Antibody (SC05-85) (Product # MA5-32191) shows increased expression of JNK2 in Raji, HeLa, NIH/3T3 cell lines when compared to HL-60. {RE}

产品信息

MA5-32191

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:50-1:200

流式细胞分析 (Flow)

1:50-1:100

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

SC05-85

抗原

Synthetic peptide within Human JNK2 aa 341-394

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2809478

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

MAPK9 (JNK2) is a serine/threonine kinase which plays an important role in the MAPK pathway. JNK2 (p54a, SAPK1a), along with JNK1 and JNK3, is thought to play an important role in nuclear signal transduction through its environmental stress activation and subsequent phosphorylation of the nuclear transcription factor p53.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: c-Jun kinase 2; c-Jun N-terminal kinase 2; JNK; JNK-46; JNK-55; JNK/SAPK alpha; JNK1; JNK1A2; JNK21B1/2; Jun kinase; MAP kinase 10; MAP kinase 8; MAP kinase 9; MAPK 10; MAPK 9; MAPK10; MAPK8; mitogen activated protein kinase 9; Mitogen-activated protein kinase 9; OTTHUMP00000161542; OTTHUMP00000161543; p54-alpha; PRKM8; protein kinase, mitogen-activated 9; SAPK-alpha; SAPK1; SAPK1a; SAPK1c; stress activated protein kinase alpha II; Stress-activated protein kinase 1a; Stress-activated protein kinase JNK2

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基因别名: AI851083; JNK-55; JNK2; JNK2A; JNK2ALPHA; JNK2B; JNK2BETA; MAPK9; p54a; p54aSAPK; PRKM9; SAPK; SAPK1A

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UniProt ID: (Human) P45984, (Rat) P49186, (Mouse) Q9WTU6

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Entrez Gene ID: (Human) 5601, (Rat) 50658, (Mouse) 26420

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