Invitrogen

KCNMB1 Polyclonal Antibody

KCNMB1 Antibody in Immunocytochemistry (ICC/IF)

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KCNMB1 Antibody (PA1-924) in ICC/IF

Immunofluorescent analysis of Maxi K+ Beta (green) in Movas cells transfected with (+) and without (-) Maxi K+ Beta siRNA. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 10 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a p38 polyclonal antibody (Product # AHO1202) at a dilution of 1:100 in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (Heavy Chain) Superclona... View More

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产品信息

PA1-924

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2.0 µg/mL

查看6篇已发表文章 6篇已发表文章

免疫组化 (IHC)

查看4篇已发表文章 4篇已发表文章

免疫细胞化学 (ICC/IF)

1:100-1:200

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Dog, Human, Mouse, Rabbit, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic Peptide: L(90) Y H T E D T R D Q N Q Q C(103)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2249548

产品详细信息

PA1-924 detects large conductance, calcium-activated potassium channel beta (Maxi K+ Beta) from human, mouse, and rat samples.

PA1-924 has been successfully used in western blot and immunofluorescence. By western blot, this antibody detects an ~28kDa protein representing Maxi K+ beta from mouse myometrial smooth muscle cell extracts. The molecular weight detected varies due to glycosylation, multimerization and binding to actin.

Immunizing peptide corresponds to amino acid residues 90-103 from human Maxi K+ beta. This sequence is completely conserved across many species. PA1-924 immunizing peptide (Cat. # PEP-119) is available for use in neutralization and control experiments.

靶标信息

Potassium channels are a group of ubiquitously expressed proteins that serve numerous functions in excitable and non-excitable cells. One class of integral membrane potassium channels is the large conductance, calcium-activated potassium channel (Maxi K+). Maxi K+ differs from most other potassium channels in that its activation is controlled by both increases in intracellular calcium and by membrane depolarization. Maxi K+ dual activation is possible because of its structure. The core of the channel, which is similar to other potassium channels, is a Maxi K+ alpha homotetramer that contains both a voltage sensor and an intracellular calcium binding domain. In vascular smooth muscle, an auxiliary beta-subunit is found in a 1:1 stoichiometry. The beta-subunit exhibits its effect on the Maxi K+ channel by effectively decreasing by 5- to 10- fold the concentration of calcium required to keep the pore open. Maxi K+ beta is the target for possible therapeutics because of its role in blood flow and blood pressure regulation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Different Effects of Hypertension and Age on the Function of Large Conductance Calcium- and Voltage-Activated Potassium Channels in Human Mesentery Artery Smooth Muscle Cells.

Journal of the American Heart Association

Cheng J,Mao L,Wen J,Li PY,Wang N,Tan XQ,Zhang XD,Zeng XR,Xu L,Xia XM,Xia D,He K,Su S,Yao H,Yang Y

PA1924 was used in western blot to study the effects of hypertension and aging on the functional changes of large-conductance calcium- and voltage-activated potassium channels

Wed Sep 14 00:00:00 UTC 2016

Western blot analysis of BK channel β1-subunit expression should be interpreted cautiously when using commercially available antibodies.

Physiological reports

Bhattarai Y,Fernandes R,Kadrofske MM,Lockwood LR,Galligan JJ,Xu H

PA1-924 was used in western blot to assess the specificity and sensitivity of six commercially available antibodies to BK beta1 using tissues from wild type and KO mice

Wed Oct 01 00:00:00 UTC 2014

Function of BKCa channels is reduced in human vascular smooth muscle cells from Han Chinese patients with hypertension.

Hypertension (Dallas, Tex. : 1979)

Yang Y,Li PY,Cheng J,Mao L,Wen J,Tan XQ,Liu ZF,Zeng XR

PA1-924 was used in Western Blotting to investigate whether large-conductance Ca(2+)- and voltage-activated K(+) channels are involved in dysfunctional relaxation of artery in Han Chinese patients with hypertension.

Fri Feb 01 00:00:00 UTC 2013

Reduced vascular smooth muscle BK channel current underlies heart failure-induced vasoconstriction in mice.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Wan E,Kushner JS,Zakharov S,Nui XW,Chudasama N,Kelly C,Waase M,Doshi D,Liu G,Iwata S,Shiomi T,Katchman A,D'Armiento J,Homma S,Marx SO

PA1-924 was used in immunohistochemistry to study the role of vascular smooth muscle BK channel activity in peripheral vasoconstriction caused by heart failure

Wed May 01 00:00:00 UTC 2013

Exercise training attenuates ageing-induced BKCa channel downregulation in rat coronary arteries.

Experimental physiology

Albarwani S,Al-Siyabi S,Baomar H,Hassan MO

PA1-924 was used in western blot to investigate the influence of exercise on the expression of large-conductance voltage- and calcium-activated potassium channels in coronary arteries of old rats

Tue Jun 01 00:00:00 UTC 2010

Shear stress-induced volume decrease in C11-MDCK cells by BK-alpha/beta4.

American journal of physiology. Renal physiology

Holtzclaw JD,Liu L,Grimm PR,Sansom SC

PA1-924 was used in immunocytochemistry to investigate the role of BK-alpha/beta in the modulation of cell volume

Wed Sep 01 00:00:00 UTC 2010

Impaired function of coronary BK(Ca) channels in metabolic syndrome.

American journal of physiology. Heart and circulatory physiology

Borbouse L,Dick GM,Asano S,Bender SB,Dincer UD,Payne GA,Neeb ZP,Bratz IN,Sturek M,Tune JD

Published figure using KCNMB1 polyclonal antibody (Product # PA1-924) in Western Blot

Sun Nov 01 00:00:00 UTC 2009

Interleukin-4 activates large-conductance, calcium-activated potassium (BKCa) channels in human airway smooth muscle cells.

Experimental physiology

Martin G,O'Connell RJ,Pietrzykowski AZ,Treistman SN,Ethier MF,Madison JM

PA1-924 was used in immunocytochemistry to investigate the role of IL-4 on calcium-activated potassium (BKCa) activity in human airway smooth muscle cells

Tue Jul 01 00:00:00 UTC 2008

Altered expression of BK channel beta1 subunit in vascular tissues from spontaneously hypertensive rats.

American journal of hypertension

Chang T,Wu L,Wang R

PA1-924 was used in western blot to study the association of BK channel beta1 subunit expression with blook pressure

Sat Jul 01 00:00:00 UTC 2006

BK-{beta}1 subunit: immunolocalization in the mammalian connecting tubule and its role in the kaliuretic response to volume expansion.

American journal of physiology. Renal physiology

Pluznick JL,Wei P,Grimm PR,Sansom SC

PA1-924 was used in immunohistochemistry to characterize calcium-activated potassium channels in the mammalian connecting tubule

Fri Apr 01 00:00:00 UTC 2005

Somatic localization of a specific large-conductance calcium-activated potassium channel subtype controls compartmentalized ethanol sensitivity in the nucleus accumbens.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Martin G,Puig S,Pietrzykowski A,Zadek P,Emery P,Treistman S

PA1-924 was used in immunohistochemistry to investigate the role of large-conductance calcium-activated potassium channels in alcohol addiction .

Wed Jul 21 00:00:00 UTC 2004

Downregulation of the BK channel beta1 subunit in genetic hypertension.

Circulation research

Amberg GC,Santana LF

PA1-924 was used in immunohistochemistry to investigate BK channels' functional properties in a rat model of genetic hypertension

Fri Nov 14 00:00:00 UTC 2003

生物信息学

蛋白别名: big potassium channel beta subunit 1; BK channel beta subunit; BK channel beta subunit 1; BK channel subunit beta-1; BKbeta; calcium-activated potassium channel beta subunit; Calcium-activated potassium channel subunit beta; Calcium-activated potassium channel subunit beta-1; Calcium-activated potassium channel, subfamily M subunit beta-1; Charybdotoxin receptor subunit beta-1; K(VCA)beta-1; large conductance Ca2+-activated K+ channel beta 1 subunit; Maxi K channel subunit beta-1; MaxiK channel beta-subunit 1; potassium channel subfamily M regulatory beta subunit 1; potassium large conductance calcium-activated channel, subfamily M, beta member 1; Slo-beta; Slo-beta-1; Slowpoke-beta

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基因别名: BKbeta; BKbeta1; hbeta1; hslo-beta; K(VCA)beta; k(VCA)beta-1; KCNMB1; SLO-BETA; slo-beta-1

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UniProt ID: (Human) Q16558, (Rat) P97678, (Mouse) Q8CAE3

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Entrez Gene ID: (Human) 3779, (Rat) 29747, (Mouse) 16533

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功能

calcium-activated potassium channel activity potassium channel regulator activity

参与通路

detection of calcium ion chemical synaptic transmission response to calcium ion potassium ion transmembrane transport transport ion transport potassium ion transport