Invitrogen

KCNQ3 Polyclonal Antibody

KCNQ3 Antibody in Immunocytochemistry (ICC/IF)

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KCNQ3 Antibody (PA1-930) in ICC/IF

Immunofluorescence analysis of KCNQ3 was performed using 70% confluent log phase RSC-96 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with KCNQ3 Rabbit Polyclonal Antibody (Product # PA1-930) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2... View More

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产品信息

PA1-930

应用

建议稀释比

已发表文章

免疫印迹 (WB)

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免疫组化（冰冻） (IHC (F))

0.5 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

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其他PubMed (MISC)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

GST fusion protein encoding the first 71 amino acids of rat KCNQ3.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2131708

产品详细信息

PA1-930 detects KCNQ3 from mouse, human and rat samples. This antibody is specific for KCNQ3 and does not detect KCNQ1, KCNQ2, KCNQ4 or KCNQ5.

PA1-930 has been successfully used in immunofluorescence, immunohistochemistry and immunocytochemistry procedures. Immunohistochemical staining using PA1-930 yielded a strong signal mainly in interneurons and astrocytes in the dentate region of rat hippocampal samples.

PA1-930 immunogen is a GST fusion protein encoding the first 71 amino acids of rat KCNQ3.

靶标信息

KCNQs are members of the voltage-dependent non-inactivating potassium channel family. Currently there are five known KCNQs (KCNQ1-5) found in the central nervous system and KCNQ2 and 3 have demonstrated their importance in M-current activation. Studies have shown that KCNQ2 and KCNQ3 form heteromultimers that, when formed, substantially increase the M-current. Inhibition of M-current controls neuron excitability throughout the nervous system as well as the responsiveness to synaptic inputs. Genetic mutations in these proteins have been linked to disorders such as benign familial neonatal convulsions (BFNC), deafness, neuropathic pain and epilepsy.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Nine patients with KCNQ2-related neonatal seizures and functional studies of two missense variants.

Scientific reports

Chokvithaya S,Caengprasath N,Buasong A,Jantasuwan S,Santawong K,Leela-Adisorn N,Tongkobpetch S,Ittiwut C,Saengow VE,Kamolvisit W,Boonsimma P,Bongsebandhu-Phubhakdi S,Shotelersuk V

PA1-930 was used in Immunocytochemistry to demonstrate that the surface membrane expression of Kv7.2 carrying either variant was decreased.

Mon Feb 27 00:00:00 UTC 2023

A novel homozygous KCNQ3 loss-of-function variant causes non-syndromic intellectual disability and neonatal-onset pharmacodependent epilepsy.

Epilepsia open

Lauritano A,Moutton S,Longobardi E,Tran Mau-Them F,Laudati G,Nappi P,Soldovieri MV,Ambrosino P,Cataldi M,Jouan T,Lehalle D,Maurey H,Philippe C,Miceli F,Vitobello A,Taglialatela M

PA1-930 was used in Western Blotting to indicate that a homozygous KCNQ3 loss-of-function variant is responsible for a severe phenotype characterized by neonatal-onset pharmacodependent seizures, with developmental delay and intellectual disability.

Sun Sep 01 00:00:00 UTC 2019

Loss of the m-AAA protease subunit AFG₃L₂ causes mitochondrial transport defects and tau hyperphosphorylation.

The EMBO journal

Kondadi AK,Wang S,Montagner S,Kladt N,Korwitz A,Martinelli P,Herholz D,Baker MJ,Schauss AC,Langer T,Rugarli EI

Published figure using KCNQ3 polyclonal antibody (Product # PA1-930) in Immunocytochemistry

Fri May 02 00:00:00 UTC 2014

Antibodies and a cysteine-modifying reagent show correspondence of M current in neurons to KCNQ2 and KCNQ3 K+ channels.

British journal of pharmacology

Roche JP,Westenbroek R,Sorom AJ,Hille B,Mackie K,Shapiro MS

PA1-930 was used in immunocytochemistry and immunohistochemistry to investigate the influence of KCNQ2 and KCNQ3 potassium channels on the M current of neurons

Sun Dec 01 00:00:00 UTC 2002

生物信息学

蛋白别名: BFNC; KQT-like 3; Potassium channel subunit alpha KvLQT3; potassium channel, voltage gated KQT-like subfamily Q, member 3; potassium channel, voltage-gated, subfamily Q, member 3; Potassium voltage-gated channel subfamily KQT member 3; potassium voltage-gated channel, KQT-like subfamily, member 3; potassium voltage-gated channel, subfamily Q, member 3; Voltage-gated potassium channel subunit Kv7.3

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基因别名: BFNC2; EBN2; KCNQ3; KV7.3

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UniProt ID: (Human) O43525, (Rat) O88944, (Mouse) Q8K3F6

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Entrez Gene ID: (Human) 3786, (Rat) 29682, (Mouse) 110862

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功能

voltage-gated potassium channel activity delayed rectifier potassium channel activity potassium channel activity calmodulin binding protein kinase binding ubiquitin protein ligase binding ion channel binding ion channel activity voltage-gated ion channel activity

参与通路