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KDM5A Antibody (PA5-121081) in WB
Western blot analysis of KDM5A in extracts of various cell lines. Samples were incubated with KDM5A Polyclonal antibody (Product # PA5-121081) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
产品信息
PA5-121081
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein of human KDM5A
偶联物
形式
Liquid
浓度
0.66 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2914653
产品详细信息
Positive test controls include: Jurkat, HeLa, MCF7. The target is usually found in the following locations: Nucleus, nucleolus.
靶标信息
UPF3B is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The protein is one of two functional homologs to yeast Upf3p. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctionsThis gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: [histone H3]-trimethyl-L-lysine(4) demethylase 5A; Histone demethylase JARID1A; jumonji, AT rich interactive domain 1A (Rbp2 like); Jumonji, AT rich interactive domain 1A (RBP2-like); Jumonji/ARID domain-containing protein 1A; lysine (K)-specific demethylase 5A; Lysine-specific demethylase 5A; RBBP-2; retinoblastoma binding protein 2; Retinoblastoma-binding protein 2
基因别名: JARID1A; KDM5A; RBBP-2; RBBP2; RBP2
UniProt ID: (Human) P29375
Entrez Gene ID: (Human) 5927, (Rat) 312678
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
protein binding
zinc ion binding
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
chromatin DNA binding
histone demethylase activity
molecular_function
chromatin binding
histone modifying enzyme
negative regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
spermatogenesis
male gonad development
circadian regulation of gene expression
histone H3-K4 demethylation
positive regulation of transcription, DNA-templated
oxidation-reduction process
negative regulation of histone deacetylase activity
regulation of nucleic acid-templated transcription
