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KEAP1 Antibody (PA5-88141) in ICC/IF
Immunofluorescence analysis of Kelch-like ECH-associated protein 1 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.01% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with KEAP1 Polyclonal Antibody (Product # PA5-88141) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor... View More
产品信息
PA5-88141
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence within amino acids 301-400 of human KEAP1 (NP_0364212)
偶联物
形式
Liquid
浓度
0.268 mg/mL
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2804684
产品详细信息
Positive Samples: MCF7, U-87MG, Jurkat; Cellular Location: Cytoplasm, Nucleus
Immunogen sequence: LVKIFEELTL HKPTQVMPCR APKVGRLIYT AGGYFRQSLS YLEAYNPSDG TWLRLADLQV PRSGLAGCVV GGLLYAVGGR NNSPDGNTDS SALDCYNPMT
靶标信息
This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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