Invitrogen

KGF Polyclonal Antibody, PeproTech®

3 篇参考文献

查看其他11个KGF抗体

抗体检测数据 (3)

KGF Antibody in Western Blot (WB)

KGF Antibody in Western Blot (WB)

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KGF Antibody (500-P19-1MG) in WB

Western Blot: To detect Human KGF (FGF-7) by Western Blot analysis KGF Polyclonal Antibody (Product # 500-P19-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human KGF (FGF-7) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

KGF Antibody in Western Blot (WB)

KGF Antibody in Western Blot (WB)

KGF Antibody in ELISA (ELISA)

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KGF Antibody in Western Blot (WB) — Western Blot: To detect Human KGF (FGF-7) by Western Blot analysis KGF Polyclonal Antibody (Product # 500-P19-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human KGF (FGF-7) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

KGF Antibody in Western Blot (WB) — Western Blot: To detect Human KGF (FGF-7) by Western Blot analysis KGF Polyclonal Antibody (Product # 500-P19-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human KGF (FGF-7) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

KGF Antibody in ELISA (ELISA) — Sandwich ELISA: To detect Human KGF (FGF-7) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of KGF Polyclonal Antibody (Product # 500-P19-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech KGF Polyclonal Antibody, Biotin (Product # 500-P19BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human KGF (FGF-7).

产品信息

500-P19-1MG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

-

酶联免疫吸附实验 (ELISA)

0.5-2.0 µg/mL

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中和实验 (Neu)

-

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免疫染色 (IS)

-

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产品规格

种属反应

Human

已发表种属

Human, Rat

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Human KGF (FGF-7)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

AB_2929754

产品详细信息

AA Sequence of recombinant protein: CNDMTPEQMA TNVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNNYNIMEIR TVAVGIVAIK GVESEFYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHNGG EMFVALNQKG IPVRGKKTKK EQKTAHFLPM AIT.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human KGF (FGF-7). Anti-Human KGF (FGF-7)-specific antibody was purified by affinity chromatography employing an immobilized Human KGF (FGF-7) matrix.

Sandwich ELISA: To detect Human KGF (FGF-7) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human KGF (FGF-7) (500-P19Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human KGF (FGF-7).

Western Blot: To detect Human KGF (FGF-7) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human KGF (FGF-7) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

靶标信息

Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A novel solid-phase site-specific PEGylation enhances the in vitro and in vivo biostabilty of recombinant human keratinocyte growth factor 1.

PloS one

Huang Z,Zhu G,Sun C,Zhang J,Zhang Y,Zhang Y,Ye C,Wang X,Ilghari D,Li X

Mon Sep 10 00:00:00 UTC 2012

Regulation of epithelial-mesenchymal IL-1 signaling by PPARbeta/delta is essential for skin homeostasis and wound healing.

The Journal of cell biology

Chong HC,Tan MJ,Philippe V,Tan SH,Tan CK,Ku CW,Goh YY,Wahli W,Michalik L,Tan NS

Mon Mar 23 00:00:00 UTC 2009

Regulation of epithelial-mesenchymal IL-1 signaling by PPARbeta/delta is essential for skin homeostasis and wound healing.

The Journal of cell biology

Chong HC,Tan MJ,Philippe V,Tan SH,Tan CK,Ku CW,Goh YY,Wahli W,Michalik L,Tan NS

Mon Mar 23 00:00:00 UTC 2009

生物信息学

蛋白别名: FGF-7; Fibroblast growth factor 7; HBGF-7; Heparin-binding growth factor 7; Keratinocyte growth factor

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基因别名: FGF7; HBGF-7; KGF

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UniProt ID: (Human) P21781

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Entrez Gene ID: (Human) 2252

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protein tyrosine kinase activity Ras guanyl-nucleotide exchange factor activity type 2 fibroblast growth factor receptor binding protein binding growth factor activity heparin binding 1-phosphatidylinositol-3-kinase activity chemoattractant activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity growth factor intercellular signal molecule

MAPK cascade ovarian follicle development positive regulation of defense response to virus by host phosphatidylcholine biosynthetic process signal transduction positive regulation of cell proliferation fibroblast growth factor receptor signaling pathway epidermis development response to wounding mesenchymal cell proliferation positive regulation of keratinocyte proliferation regulation of phosphatidylinositol 3-kinase signaling peptidyl-tyrosine phosphorylation hair follicle morphogenesis actin cytoskeleton reorganization protein localization to cell surface phosphatidylinositol-3-phosphate biosynthetic process wound healing surfactant homeostasis positive regulation of GTPase activity positive regulation of transcription, DNA-templated phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling positive regulation of epithelial cell proliferation positive regulation of peptidyl-tyrosine phosphorylation positive chemotaxis positive regulation of keratinocyte migration positive regulation of cell division branching involved in salivary gland morphogenesis positive regulation of epithelial cell proliferation involved in lung morphogenesis regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling secretion by lung epithelial cell involved in lung growth xenophagy