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KIF11 Antibody (MA5-32792) in ICC/IF
Immunofluorescence analysis of Kinesin-like protein KIF11 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with KIF11 Recombinant Rabbit Monoclonal Antibody (JU83-16) (Product # MA5-32792) at 1:100 dilution (green channel) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross... View More
产品信息
MA5-32792
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JU83-16
抗原
Recombinant protein within Human Eg5 aa 860-1,056
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2810068
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Kines; Kinesin-like protein 1; Kinesin-like protein KIF11; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; Thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP-5
基因别名: EG5; HKSP; KIF11; KNSL1; MCLMR; TRIP5
UniProt ID: (Human) P52732
Entrez Gene ID: (Human) 3832
retrograde vesicle-mediated transport, Golgi to ER
microtubule-based movement
spindle organization
mitotic spindle organization
chromosome segregation
mitotic nuclear division
mitotic centrosome separation
antigen processing and presentation of exogenous peptide antigen via MHC class II
regulation of mitotic centrosome separation
cell division
mitotic spindle assembly
