Invitrogen

KIF4A Polyclonal Antibody

KIF4A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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KIF4A Antibody (PA5-30492) in IHC (P)

Immunohistochemical analysis of paraffin-embedded TOV-112D xenograft, using KIF4A (Product # PA5-30492) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Product Image Gallery

KIF4A Antibody — Polar Ejection Forces on Chromosome Arms Ensure Mitotic Fidelity but Are Not Implicated in the Observed Missegregation Bias for Chromosomes with Large Kinetochores (A) Live-cell imaging of Indian muntjac fibroblasts stably expressing H2B-GFP to visualize the chromosomes (green) in control (top) and Kif4a RNAi (bottom) cells treated with 50 nM SiR-tubulin to label spindle microtubules (magenta). Scale bar, 5 µm. Time, hr:min. White arrows point to the chromosome arms facing the spindle poles. (B) Western blot to monitor Kif4a levels after RNAi. GAPDH was used as loading control. (C) Chromosome missegregation after Kif4a RNAi (fixed cells). Kinetochores (anti-ACA), α-tubulin, and DNA (DAPI) are indicated. Scale bars, 5 µm. (D and E) Comparison of the frequency of anaphase cells with lagging chromosomes in live (D) and fixed (E) material after Kif4a depletion and/or monastrol washout. (F) Frequency of anaphase cells with at least 1 lagging chromosome with small or large kinetochores after monastrol washout in Kif4a-depleted fibroblasts. Dashed bar represents theoretical values for frequencies of lagging chromosomes with small kinetochores if the probability to lag was equal for chromosomes with small or large kinetochores. {KD}

产品信息

PA5-30492

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 50 and 305 of Human KIF4A

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2547966

产品详细信息

Recommended positive controls: HeLa, HepG2, HCT116, 293T, A431.

Predicted reactivity: Xenopus laevis (80%), Chicken (81%), Bovine (96%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene encodes a member of the kinesin 4 or subfamily of kinesin related proteins. This subfamily is also referred to as the chromokinesins. The encoded protein is an ATP dependent microtubule-based motor protein that is involved in the intracellular transport of membranous organelles. This protein also associates with condensed chromosome arms and may be involved maintaining chromosome integrity during mitosis. This protein may also be involved in the organization of the central spindle prior to cytokinesis. A pseudogene of this gene is found on chromosome X.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The kinesin KIF4 mediates HBV/HDV entry through the regulation of surface NTCP localization and can be targeted by RXR agonists in vitro.

PLoS pathogens

Gad SA,Sugiyama M,Tsuge M,Wakae K,Fukano K,Oshima M,Sureau C,Watanabe N,Kato T,Murayama A,Li Y,Shoji I,Shimotohno K,Chayama K,Muramatsu M,Wakita T,Nozaki T,Aly HH

PA5-30492 was used in Western Blot to show that human Kinesin Family Member 4 (KIF4) is a critical regulator of sodium taurocholate cotransporting polypeptide (NTCP) surface transport and localization, which is required for NTCP to function as a receptor for B and delta hepatitis virus entry.

Tue Mar 01 00:00:00 UTC 2022

The kinesin KIF4 mediates HBV/HDV entry through the regulation of surface NTCP localization and can be targeted by RXR agonists in vitro.

PLoS pathogens

Gad SA,Sugiyama M,Tsuge M,Wakae K,Fukano K,Oshima M,Sureau C,Watanabe N,Kato T,Murayama A,Li Y,Shoji I,Shimotohno K,Chayama K,Muramatsu M,Wakita T,Nozaki T,Aly HH

Tue Mar 01 00:00:00 UTC 2022

An integrated functional and clinical genomics approach reveals genes driving aggressive metastatic prostate cancer.

Nature communications

Das R,Sjöström M,Shrestha R,Yogodzinski C,Egusa EA,Chesner LN,Chen WS,Chou J,Dang DK,Swinderman JT,Ge A,Hua JT,Kabir S,Quigley DA,Small EJ,Ashworth A,Feng FY,Gilbert LA

PA5-30492 was used in Western Blot to investigate if both KIF4A and WDR62 drive aggressive prostate cancer phenotypes in vitro and in vivo in multiple models, irrespective of AR-status, and if they are also associated with poor patient outcome.