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KLF4 Antibody (PA1-095) in ICC/IF
Immunofluorescent analysis of KLF4 (red) in human embryonic stem cell H9 line grown on irradiated MEF-feeder layer. The cells were fixed with 4% paraformaldehyde at room temperature for 10 min and permeabilized with 0.25% Triton-X 100 for 5 min and blocked with the 10% BSA in PBS for 30 min at 37°C. Cells were stained with a KLF4 polyclonal antibody (Product # PA1-095) at a dilution of 1:200 in 3% BSA/PBS blocking buffer overnight at 4°C, and then incubated with a RRX-conjugated donkey anti-rabbit IgG secondary antibody at a dilution of 1:... View More
产品信息
PA1-095
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant N-terminal fragment (amino acids 1-118) of human KLF4
偶联物
形式
Liquid
浓度
0.5 mg/mL
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2539864
产品详细信息
The predicted molecular weight of KLF4 is ~55kD. The migration of this protein by SDS-PAGE was observed slightly higher (~60kD) with both PA1-095 and a benchmark antibody.
PA1-095 detects a predominant band at ~60kD, and a nonspecific band of unknown origin at ~80kD in both whole cell lysates and nuclear extracts.
For best results, StartingBlock T20 (TBS) Blocking Buffer (Product # 37543) is recommended.
靶标信息
Kruppel like factor 4 (KLF4) protein is encoded by the KLF4 gene located on chromosome 9 in humans. It is also known as gut-enriched Kruppel-like factor (GKLF) or epithelial zinc-finger protein (EZF) and belongs to a family of conserved Kruppel-like factor family of DNA binding zinc finger transcription factors. KLF4 exerts its functional relevance across varied cellular pathways. As one of the four Yamanaka factors, it plays a key role in reprogramming differentiated cells into pluripotent stem cells. Most studies have focused on its specific role in endothelial cell signaling, adipogenesis, differentiation, embryogenesis and cell cycle control. Localization plays an important role in the biological significance of this protein wherein nuclear localization is associated with its function as an oncogene and cytosolic localization with tumor suppression specific effect.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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